Ketamine inhibits monoamine transporters expressed in human embryonic kidney 293 cells
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Background: Ketamine has been characterized as having psychotomimetic and sympathomimetic effects. These symptoms have raised the possibility that ketamine affects monoaminergic neurotransmission. To elucidate the relation between ketamine and monoamine transporters, the authors constructed three cell lines that stably express the norepinephrine, dopamine, and serotonin transporters and investigated the effects of ketamine on these transporters. Methods: Human embryonic kidney cells were transfected using the Chen-Okayama method with the human norepinephrine, rat dopamine, and rat serotonin transporter cDNA subcloned into the eukaryotic expression vector. Using cells stably expressing these transporters, the authors investigated the effects of ketamine on the uptake of these compounds and compared them with those of pentobarbital. Results: Inhibition analysis showed that ketamine significantly inhibited the uptake of all three monoamine transporters in a dose-dependent manner. The Ki (inhibition constant) values of ketamine on the norepinephrine, dopamine, and serotonin transporters were 66.8 microM, 62.9 microM, and 162 microM, respectively. Pentobarbital, a typical general anesthetic agent with no psychotic symptoms, did not affect the uptake of monoamines, however. Further, neither the glycine transporter 1 nor the glutamate/aspartate transporter was affected by ketamine, indicating that ketamine preferentially inhibits monoamine transporters. Conclusions: Ketamine inhibited monoamine transporters expressed in human embryonic kidney cells in a dose-dependent manner. This result suggests that the ketamine-induced inhibition of monoamine transporters might contribute to its psychotomimetic and sympathomimetic effects through potentiating monoaminergic neurotransmission.
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At high concentrations, ketamine inhibited NET, DAT, and SERT with Ki values of 66.8, 62.9, and 162 μM, respectively.
“it was found that high concentrations of ketamine (Ki = 66.8, 62.9, and 162 μM) can functionally inhibit NET, DAT, and SERT respectively (Nishimura et al., 1998).”
From Mechanisms of ketamine and its metabolites as antidepressants (2022) · cites this paper as Nishimura et al., 1998
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- Authors
- Nishimura, M., Sato, K., Okada, T., Yoshiya, I., Schloss, P., Shimada, S., & Tohyama, M.
- Affiliations
- Osaka University Hospital (JP)
- The University of Osaka (JP)
- Max Planck Society (DE)
- Venue
- Anesthesiology
- Published
- 1998-03-01
- Volume
- 88
- Issue
- 3
- Pages
- 768-774
- ISSN
- 0003-3022 (ISSN-L)
- OpenAlex
- W2091155334
- Cited by
- 128
- References
- 20
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 5 · 2023: 5 · 2022: 4 · 2021: 11 · 2020: 10 · 2019: 11 · 2018: 5 · 2017: 11 · 2016: 11 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 4
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Anesthesiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- ketamine, monoamine transporters, norepinephrine transporter, dopamine transporter, serotonin transporter, psychotomimetic effects, sympathomimetic effects, monoaminergic neurotransmission, transporter inhibition, pentobarbital, glycine transporter 1, GLAST, general anesthetics
- MeSH terms
- Anesthetics, Dissociative, Animals, Biological Transport, Carrier Proteins, Cell Line, Humans, Ketamine, Membrane Glycoproteins, Nerve Tissue Proteins, Recombinant Proteins, ATP-Binding Cassette Transporters, Membrane Transport Proteins, Amino Acid Transport Systems, Neutral, Amino Acid Transport System X-AG, Symporters, Dopamine Plasma Membrane Transport Proteins, Norepinephrine Plasma Membrane Transport Proteins, Serotonin Plasma Membrane Transport Proteins, Glycine Plasma Membrane Transport Proteins, Rats
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