Generation of Aldehyde‐Derived Protein Modifications in Ethanol‐Exposed Heart
Abstract
Background: Although excessive ethanol consumption is known to lead to a variety of adverse effects in the heart, the molecular mechanisms of such effects have remained poorly defined. We hypothesized that posttranslational covalent binding of reactive molecular species to proteins occurs in the heart in response to acute ethanol exposure. Methods: The generation of protein adducts with several aldehydic species was examined by using monospecific antibodies against adducts with malondialdehyde (MDA), acetaldehyde (AA), MDA‐AA hybrids, and hydroxyethyl radicals. Specimens of heart tissue were obtained from rats after intraperitoneal injections with alcohol (75 mmol/kg body weight) with or without pretreatment with cyanamide (0.05 mmol/kg body weight), an aldehyde dehydrogenase inhibitor. Results: The amounts of MDA and unreduced AA adducts were found to be significantly increased in the heart of the rats treated with ethanol, cyanamide, or both, whereas no other adducts were detected in statistically significant quantities. Immunohistochemical studies for characterization of adduct distribution revealed sarcolemmal adducts of both MDA and AA in the rats treated with ethanol and cyanamide in addition to intracellular adducts, which were also present in the group treated with ethanol alone. Conclusions: These findings support the role of enhanced lipid peroxidation and the generation of protein‐aldehyde condensates in vivo as a result of excessive ethanol intake. These findings may have implications in the molecular mechanisms of cardiac dysfunction in alcoholics.
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Elevated oxidative stress caused by alcohol oxidation increases acetaldehyde and MDA adduct synthesis in the heart.
“Elevated oxidative stress caused by alcohol oxidation increases acetaldehyde and MDA adduct synthesis in the heart243,244.”
From Advanced glycation end products (AGEs) and other adducts in aging-related diseases and alcohol-mediated tissue injury (2021) · cites this paper as [243]
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Recent Advances in Alcohol‐Induced Adduct Formation
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Self-citation
- Authors
- Niemelä, O., Parkkila, S., Worrall, S., Emery, P. W., & Preedy, V. R.
- Affiliations
- The University of Queensland (AU)
- Corresponding author
- Niemelä, Onni
- Venue
- Alcoholism: Clinical and Experimental Research
- Publisher
- Wiley
- Published
- 2003-12
- Volume
- 27
- Issue
- 12
- Pages
- 1987-1992
- ISSN
- 0145-6008 (ISSN-L)
- 1530-0277
- OpenAlex
- W2009972815
- Cited by
- 24
- References
- 61
- Citations by year
- 2024: 1 · 2021: 1 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Alcoholism Clinical and Experimental Research (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- Funders
- University of Nebraska Medical Center
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- ethanol, heart, malondialdehyde, acetaldehyde, protein adducts, lipid peroxidation, aldehyde dehydrogenase inhibition, cyanamide, sarcolemma, post-translational modification
- MeSH terms
- Ethanol, Aldehydes, Animals, Male, Myocardium, Rabbits, Rats, Wistar, Rats
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