Immunohistochemical Study on Acetaldehyde Adducts in Alcohol‐Fed Mice
Abstract
Background: Acetaldehyde binds to some proteins, which results in Schiff base formation. It is assumed that acetaldehyde binds to the proteins after the consumption of ethanol, to form an adduct. Such acetaldehyde adducts are related to organ disease. Methods: We examined 8‐week‐old male BALB/c mice, which were given a liquid diet for 7 days. The diet consisted of vitamins, minerals, amino acids, and a 5% (v/v) ethanol solution. After the 7 days, we took tissue samples from the brain, liver, and adrenal cortex to investigate the distribution of acetaldehyde adducts. We performed immunohistochemical staining of the cerebral cortex, liver, and adrenal cortex from the mice by using antibodies against acetaldehyde adducts. Results: Our study showed that acetaldehyde adducts formed in the cerebral cortex in the early phase in alcohol‐fed mice. Conclusions: Because acetaldehyde in the liver has been shown to cause liver damage, our study suggests a relationship between acetaldehyde adducts in the brain and brain damage.
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Reactive electrophilic acetaldehyde can strongly induce covalent adduct formation with nucleophilic molecules such as proteins and DNA, forming AA-protein or AA-DNA adducts in the brain.
“Additionally, reactive electrophilic acetaldehyde can strongly induce covalent adduct formation with nucleophilic molecules such as proteins and DNA, forming acetaldehyde-protein (AA-protein) or acetaldehyde-DNA (AA-DNA) adducts in the brain187–189.”
From Advanced glycation end products (AGEs) and other adducts in aging-related diseases and alcohol-mediated tissue injury (2021) · cites this paper as [188]
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- Authors
- Nakamura, K., Iwahashi, K., Itoh, M., Ameno, K., Ijiri, I., Takeuchi, Y., & Suwaki, H.
- Affiliations
- Azabu University (JP)
- Department of Forensic Medicine, Faculty of Medicine Kagawa Medical University, Miki‐cho Kita‐gun Kagawa Japan
- Corresponding author
- Iwahashi, Kazuhiko
- Venue
- Alcoholism: Clinical and Experimental Research
- Publisher
- Wiley
- Published
- 2000-04
- Volume
- 24
- Issue
- S4
- Pages
- 93S-96S
- ISSN
- 0145-6008 (ISSN-L)
- 1530-0277
- OpenAlex
- W2431376765
- Cited by
- 33
- References
- 27
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2012: 1
- FWCI
- 3.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Alcoholism Clinical and Experimental Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Health Sciences › Medicine › Neurology › Alcoholism and Thiamine Deficiency
- Health Sciences › Medicine › Epidemiology › Substance Abuse Treatment and Outcomes
- Keywords
- acetaldehyde adducts, acetaldehyde, alcohol consumption, ethanol, Schiff base formation, cerebral cortex, brain damage, liver injury, adrenal cortex
- MeSH terms
- Acetaldehyde, Adrenal Cortex, Ethanol, Animals, Brain, Brain Chemistry, Immunohistochemistry, Liver, Male, Mice, Inbred BALB C, Protein Binding, Mice
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