Effect of Recombinant Platelet-Derived Growth Factor (Regranex®) on Wound Closure in Genetically Diabetic Mice
Abstract
Burns, especially those involving large surface areas, represent a complex wound healing problem. Platelet-derived growth factor (PDGF) is released by activated platelets to recruit inflammatory cells toward the wound bed. It has effects on promoting angiogenesis and granulation tissue formation. However, the effectiveness of topical PDGF on wound closure is variable, ranging from little improvement observed in pig models to dramatic improvement reported in a diabetic mouse model. Here, we sought to determine the effectiveness of commercially sold PDGF-BB (Regranex) on wound closure in genetically diabetic mice. C57BL/KsJ db+/db+ mice and its host strain bearing dorsal 1.5-cm wounds were divided into groups (n = 8 in each group) receiving topical application of either Regranex (10 microg/wound) or vehicle for 5 consecutive days after wounding. The rate of wound closure was analyzed using computerized planimetry. The amount of granulation tissue was determined histologically. Our data indicate that diabetic mice exhibit a significant delay in wound closure when compared with their host strain. Topical application of Regranex did not improve the time to wound closure but did significantly increase the amount of granulation tissue. Our current study using commercially available Regranex failed to reproduce the previously reported finding that PDGF improved wound closure in healing impaired genetically diabetic mice.
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Despite limitations, mouse models have been instrumental in preclinical testing, leading to clinical trials and approval of products such as Regranex (PDGF-BB).
“Despite the limitations of mouse models, they have been instrumental in pre-clinical testing, leading to clinical trials and approval of products such as Regranex (PDGF-BB) (62, 115, 116).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [116]
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Wound repair and regeneration: Mechanisms, signaling, and translation
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- Authors
- Chan, R. K., Liu, P. H., Pietramaggiori, G., Ibrahim, S. I., Hechtman, H. B., & Orgill, D. P.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Orgill, Dennis P.
- Venue
- Journal of Burn Care & Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-03
- Volume
- 27
- Issue
- 2
- Pages
- 202-205
- ISSN
- 1559-047X (ISSN-L)
- OpenAlex
- W2049524851
- Cited by
- 88
- References
- 12
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 7 · 2020: 5 · 2019: 3 · 2018: 8 · 2017: 2 · 2016: 6 · 2015: 5 · 2014: 10 · 2013: 4 · 2012: 2
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Burn Care & Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
- Health Sciences › Medicine › Epidemiology › Burn Injury Management and Outcomes
- Keywords
- platelet-derived growth factor, PDGF-BB, wound closure, diabetic wound healing, diabetic mice, granulation tissue, burns, topical growth factor therapy
- MeSH terms
- Becaplermin, Administration, Topical, Animals, Diabetes Mellitus, Experimental, Male, Mice, Inbred C57BL, Platelet-Derived Growth Factor, Time Factors, Wound Healing, Wounds, Nonpenetrating, Proto-Oncogene Proteins c-sis, Angiogenesis Inducing Agents, Mice
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