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Cited by 32Bone health and osteoporosis researchBone Metabolism and DiseasesEstrogen and related hormone effects

Different doses of nitric oxide donor prevent osteoporosis in ovariectomized rats

Hao, Ying-Jie, Tang, Yu, Chen, Feng-Bao, Pei, Fu-Xing·2005·Clinical Orthopaedics and Related Research
DOI 10.1097/01.blo.0000153990.74837.73
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Abstract

Nitric oxide has important effects on bone cell function. To verify that nitric oxide can protect against bone loss associated with estrogen deficiency, which is dependent on different concentrations of nitric oxide, we applied different doses of nitric oxide to ovariectomized rats. Fifty 12-week-old Sprague-Dawley female rats had ovariectomies, and 10 rats had sham operations. The ovariectomized rats were randomized into five groups: ovariectomized only; 17-beta-estradiol; low-dose nitroglycerin; middle-dose nitroglycerin; and high-dose nitroglycerin. After 12 weeks, the bone mineral density, dry weight, ash weight, calcium content, and nitric oxide concentration were determined. Compared with these same measurements in the sham-operated group, the bone mineral density, dry weight, ash weight, calcium content, and nitric oxide concentration decreased in the control group. Treatment with low-dose nitroglycerin, middle-dose nitroglycerin, and 17-beta-estradiol maintained bone mineral density and reversed the effects of ovariectomy on dry weight, ash weight and calcium content when compared with those in the control group. There were no differences in the bone mineral density, dry weight, ash weight, or calcium concentration between the ovariectomized-only rats and the rats treated with high-dose nitroglycerin. Results of this study suggest that nitric oxide treatment can counteract bone loss in ovariectomized rats. Furthermore, supplementation with a similar or slightly greate…

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Crossref · 10.1097/01.blo.0000153990.74837.73

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  • Organic nitrate NO donors such as glycerol trinitrate, isosorbide dinitrate, and mononitrate all have beneficial effects on experimental and clinical osteoporosis, and a number of epidemiological studies indicated that high fruit and vegetable intake appears protective against osteoporosis in men and pre- and postmenopausal women.

    “In this context, it has been shown that organic nitrate NO donors, such as glycerol trinitrate, isosorbide dinitrate and mononitrate all have beneficial effects on experimental and clinical osteoporosis [161,162,163], and a number of epidemiological studies also indicated that a high fruit and vegetable intake appears to have a protective effect against osteoporosis in men and pre- and post”

    From NO-Rich Diet for Lifestyle-Related Diseases (2015) · cites this paper as [162]

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Authors
Hao, Y.-J., Tang, Y., Chen, F.-B., & Pei, F.-X.
Affiliations
  • Sichuan University (CN)
  • Zhengzhou University (CN)
Corresponding author
  • Hao, Ying-Jie
  • Pei, Fu-Xing
ORCID
Venue
Clinical Orthopaedics and Related Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2005-06
Volume
&NA;
Issue
435
Pages
226-231
ISSN
0009-921X (ISSN-L)
DOI
10.1097/01.blo.0000153990.74837.73
OpenAlex
W2077169253
Cited by
32
References
27
Citations by year
2025: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
FWCI
2.57
Type
journal article
Language
en
Open access
closed
Locations
  • Clinical Orthopaedics and Related Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
nitric oxide, nitroglycerin, osteoporosis, ovariectomy, estrogen deficiency, bone mineral density, bone loss, estradiol, dose-dependent effects, bone calcium content
MeSH terms
Analysis of Variance, Animals, Estradiol, Female, Nitroglycerin, Osteoporosis, Ovariectomy, Random Allocation, Bone Density, Rats, Sprague-Dawley, Nitric Oxide Donors, Rats

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