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Cited by 189Bone Metabolism and DiseasesBone health and osteoporosis researchEstrogen and related hormone effects

Nitric oxide donor alleviates ovariectomy-induced bone loss

Wimalawansa, S.J, De Marco, G, Gangula, P, Yallampalli, C·1996·Bone
DOI 10.1016/8756-3282(96)00005-1
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Abstract

Nitric oxide (NO) has been reported to inhibit osteoclastic bone resorption. We examined the bone-sparing effect of NO after 6 weeks of administration into estrogen-deficient rats. 30 female Sprague-Dawley rats, 12 weeks of age, underwent ovariectomy (OVX), and 5 rats were sham-operated. OVX rats were assigned to six groups (n = 5/group) treated respectively with: vehicle; 17-beta-estradiol (E2); nitroglycerine (NG, NO donor); NG-nitro-L-arginine methyl ester (L-NAME, NO synthase inhibitor); combination of E2 + NG; and a combination of E2 + L-NAME. Prior to treatment and at the end of the treatment period, bone mineral density (BMD) of rats was measured by dual-energy X-ray absorptiometry (DXA) scanning. OVX animals had significantly lower BMD and femur weights in comparison to sham operated rats (p < 0.01), and this was completely prevented by the administration of E2 (p < 0.01). Administration of NG alone prevented OVX-induced bone loss (p < 0.05). The combination of E2 + NG did not further enhance the bone mass or femur weight, and the OVX-induced bone loss was not further aggravated by L-NAME. However, in the presence of L-NAME, E2 was totally ineffective in reversing the bone loss, suggesting that the protective effect of estrogens against bone loss may be mediated through NO. In summary, the results suggest that NO counteracts the bone loss associated with OVX.

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Crossref · 10.1016/8756-3282(96)00005-1

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  • Organic nitrate NO donors such as glycerol trinitrate, isosorbide dinitrate, and mononitrate all have beneficial effects on experimental and clinical osteoporosis, and a number of epidemiological studies indicated that high fruit and vegetable intake appears protective against osteoporosis in men and pre- and postmenopausal women.

    “In this context, it has been shown that organic nitrate NO donors, such as glycerol trinitrate, isosorbide dinitrate and mononitrate all have beneficial effects on experimental and clinical osteoporosis [161,162,163], and a number of epidemiological studies also indicated that a high fruit and vegetable intake appears to have a protective effect against osteoporosis in men and pre- and post”

    From NO-Rich Diet for Lifestyle-Related Diseases (2015) · cites this paper as [161]

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Authors
Wimalawansa, S. J., De Marco, G., Gangula, P., & Yallampalli, C.
Affiliations
The University of Texas Medical Branch at Galveston (US)
Corresponding author
Wimalawansa, S.J
ORCID
Venue
Bone
Publisher
Elsevier BV
Published
1996-04
Volume
18
Issue
4
Pages
301-304
ISSN
  • 1873-2763 (ISSN-L)
  • 8756-3282
DOI
10.1016/8756-3282(96)00005-1
OpenAlex
W2071563059
Cited by
189
References
22
Citations by year
2026: 1 · 2025: 4 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 7 · 2019: 3 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 5 · 2013: 8 · 2012: 4
FWCI
3.28
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Bone (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hd030273-04)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01-HD 30273)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
nitric oxide, nitric oxide donors, nitroglycerin, ovariectomy, estrogen deficiency, bone loss, bone mineral density, osteoclast resorption, estradiol, L-NAME, nitric oxide synthase, postmenopausal osteoporosis
MeSH terms
Analysis of Variance, Animals, Arginine, Disease Models, Animal, Drug Synergism, Drug Therapy, Combination, Enzyme Inhibitors, Estradiol, Estrogens, Female, Nitroglycerin, Humans, Nitric Oxide, Ovariectomy, Absorptiometry, Photon, Bone Density, Osteoporosis, Postmenopausal, Estrogen Replacement Therapy, Rats, Sprague-Dawley, Nitric Oxide Synthase, NG-Nitroarginine Methyl Ester, Rats

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