The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Neutrophil Infiltration Increases Matrix Metalloproteinase-9 in the Ischemic Brain after Occlusion/Reperfusion of the Middle Cerebral Artery in Rats
Abstract
Matrix metalloproteinase-9 (MMP-9) activity increases in the brain during the first day after focal ischemia and might be involved in the pathogenesis of tissue damage. We previously showed MMP-9 in the extracellular space of brain parenchyma along with neutrophil recruitment after ischemia. In the present study, we tested whether neutrophils were a direct source of enhanced MMP-9 in the ischemic brain. Neutrophil infiltration was prevented either by injecting an antibody against ICAM-1, which abrogates neutrophil adhesion to the endothelial vessel wall, or by inducing neutropenia. One-hour intraluminal middle cerebral artery occlusion with reperfusion was induced, and studies were performed at 24 hours. Circulating neutrophils expressed 95-kDa MMP-9 and dimers, and infiltrated neutrophils stained positive for MMP-9. The expression of MMP-9 (mainly 95-kDa proform and dimers and, to a lesser extent, 88-kDa form) increased in brain after ischemia/reperfusion. Treatments preventing neutrophil infiltration failed to preclude the ischemia-induced increase in 88-kDa MMP-9 form and gelatinase activity in neurons and blood vessels. However, these treatments prevented the major increase in 95-kDa MMP-9 form and dimers. We conclude that neutrophil infiltration highly contributes to enhanced MMP-9 in the ischemic brain by releasing MMP-9 proform, which might participate in the tissular inflammatory reaction.
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Cited by
Chamorro, Á., Dirnagl, U., Urra, X., & Planas, A. M. (2016) · The Lancet Neurology · doi:10.1016/s1474-4422(16)00114-9 · Cited by 1,198
Self-citationCommensal microbiota affects ischemic stroke outcome by regulating intestinal γδ T cells
Benakis, C., Brea, D., Caballero, S., Faraco, G., Moore, J., Murphy, M., Sita, G., Racchumi, G., Ling, L., Pamer, E. G., et al. (2016) · Nature Medicine · doi:10.1038/nm.4068 · Cited by 1,114
Blood-brain barrier tight junction permeability and ischemic stroke
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Inflammation in stroke and focal cerebral ischemia
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Glial Cells: Role of the Immune Response in Ischemic Stroke
Xu, S., Lu, J., Shao, A., Zhang, J. H., & Zhang, J. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.00294 · Cited by 601
Metadata
- Authors
- Justicia, C., Panés, J., Solé, S., Cervera, Á., Deulofeu, R., Chamorro, Á., & Planas, A. M.
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2003-12
- Volume
- 23
- Issue
- 12
- Pages
- 1430-1440
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- OpenAlex
- W2048599333
- Cited by
- 262
- References
- 33
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- closed
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- Justicia, C., Panés, J., Solé, S., Cervera, Á., Deulofeu, R., Chamorro, Á., & Planas, A. M. (2003). Neutrophil Infiltration Increases Matrix Metalloproteinase-9 in the Ischemic Brain after Occlusion/Reperfusion of the Middle Cerebral Artery in Rats. Journal of Cerebral Blood Flow & Metabolism. https://doi.org/10.1097/01.wcb.0000090680.07515.c8
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