Discard tubes are not necessary when drawing samples for specialized coagulation testing
Abstract
Discard tubes have traditionally been obtained when drawing samples for coagulation testing to avoid potential tissue factor activation of coagulation in the first tube that may lead to inaccurate results. Although discard tubes are no longer required for prothrombin time and partial thromboplastin time, the practice is still recommended for other coagulation studies due to lack of sufficient evidence that discard tubes are not needed. The objective of the present study was to determine whether the first citrate tube drawn can be used for special coagulation testing. We performed testing for fibrinogen, D-dimer, factors VIII, IX, XI, proteins C and S, and antithrombin on 30 healthy individuals and factors II, VII, IX, X, and proteins C and S on a second group of 30 healthy individuals and 30 individuals receiving warfarin. Testing was performed on two consecutive samples to evaluate the level of agreement between the two tubes. Paired t-testing showed no statistically significant differences between tube 1 and tube 2 for any of the tests performed. Most data pairs (tube 1, tube 2) agreed within 10% difference or less, and no positive or negative biases were observed. To our knowledge, this study is the first to evaluate the need for discard tubes in a variety of coagulation tests using both normal and abnormal samples. Our data suggest that discard tubes are not necessary when drawing samples for specialized coagulation testing.
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When a coagulation tube is collected as the first or the only tube and a straight needle is used for blood collection, no discard tube is needed.
“and a straight needle is used for blood collection, no discard tube is needed [75], [76]”
From Joint EFLM-COLABIOCLI Recommendation for venous blood sampling (2018) · cites this paper as [75]
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- Authors
- Smock, K. J., Crist, R. A., Hansen, S. J., Rodgers, G. M., & Lehman, C. M.
- Affiliations
- University of Utah (US)
- ARUP Institute for Clinical and Experimental Pathology (US)
- Corresponding author
- Smock, Kristi J
- Venue
- Blood Coagulation & Fibrinolysis
- Published
- 2010-04
- Volume
- 21
- Issue
- 3
- Pages
- 279-282
- ISSN
- 0957-5235 (ISSN-L)
- OpenAlex
- W2002890223
- Cited by
- 22
- References
- 9
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood Coagulation & Fibrinolysis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Blood properties and coagulation
- Keywords
- coagulation tests, tissue factor activation, fibrinogen, D-dimer, coagulation factors, protein C, protein S, antithrombin III, warfarin
- MeSH terms
- Anticoagulants, Blood Coagulation Tests, Blood Specimen Collection, Humans, Warfarin
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