CARDIOPULMONARY BYPASS INDUCES ENDURING ALTERATIONS TO HOST NEUTROPHIL PHYSIOLOGY: A SINGLE-CENTER LONGITUDINAL OBSERVATIONAL STUDY
Abstract
Studies during and immediately post-cardiopulmonary bypass (CPB) surgery have revealed that neutrophils (PMNs) are pivotal to post-CPB inflammation and innate immunity. The aim of this study was to investigate the effects of CPB on the PMN phenotype and respiratory burst function over a longer post-CPB period (up to day 5). Blood samples were collected pre-CPB and on days 1, 3, and 5 post-CPB from 20 patients. Changes to PMN surface expression of CD16, CD62L, CD11b, CD18, and CD43, and PMN respiratory burst activity were measured, together with the white blood cell count and absolute PMN count. Cardiopulmonary bypass induced neutrophilia on days 1 and 3. One day post-CPB, CD16 expression reached a nadir (P = 0.001), and platelet-activating factor-induced CD18 increase was depressed (P < 0.05). Three days post-CPB, CD43 expression peaked (P < 0.05), with a concomitant resistance to N-formyl-Met-Leu-Phe-induced CD11b upregulation (P < 0.05). The PMN respiratory burst activity declined continuously post-CPB until day 5. Neutrophilia on days 1 and 3 was associated with changes to surface molecules expression that may reduce PMN activation response. This study demonstrated that CPB depresses the respiratory burst activity of host PMNs for an extraordinarily longer period of at least 5 days even after neutrophilia had resolved. Collectively, the changes portray an autoprotective yet responsive homeostatic balance.
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“Neutrophil-mediated organ dysfunction has been implicated as playing a causative role in the high rates of morbidity and mortality in SIRS patients [2].”
From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [2]
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“provide the most relevant information regarding immune status [2,3,21].”
Background
- Authors
- Fung, Y. L., Silliman, C. C., Minchinton, R. M., Wood, P., & Fraser, J. F.
- Affiliations
- Australian Red Cross Lifeblood (AU)
- Princess Alexandra Hospital (AU)
- Prince Charles Hospital (AU)
- Corresponding author
- Fung, Yoke Lin
- Silliman, Christopher C.
- Minchinton, Robyn M.
- Venue
- Shock
- Published
- 2008-12
- Volume
- 30
- Issue
- 6
- Pages
- 642-648
- ISSN
- 1073-2322 (ISSN-L)
- OpenAlex
- W2075255334
- Cited by
- 17
- References
- 32
- Citations by year
- 2026: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Shock (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cardiac and Coronary Surgery Techniques
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Keywords
- cardiopulmonary bypass, neutrophils, respiratory burst, neutrophilia, CD16, CD43, CD11b, CD18, L-selectin, postoperative inflammation, innate immunity, leukocyte surface markers
- MeSH terms
- Adult, Aged, Aged, 80 and over, Cardiopulmonary Bypass, Female, Flow Cytometry, Follow-Up Studies, Humans, Male, Middle Aged, Neutrophils, Respiratory Burst, CD18 Antigens, CD11b Antigen, Leukosialin
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