Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH

Abstract

Evidence is given for a high density of negative surface charge near the sodium channel of myelinated nerve fibres. The voltage dependence of peak sodium permeability is measured in a voltage clamp. The object is to measure voltage shifts in sodium activation as the following external variables are varied: divalent cation concentration and type, monovalent concentration, and pH. With equimolar substitution of divalent ions the order of effectiveness for giving a positive shift is: Ba = Sr < Mg < Ca < Co ≈; Mn < Ni < Zn. A tenfold increase of concentration of any of these ions gives a shift of + 20 to + 25 mV. At low pH, the shift with a tenfold increase in Ca2+ is much less than at normal pH, and conversely for high pH. Solutions with no added divalent ions give a shift of — 18 mV relative to 2 mM Ca2+. Removal of 7/8 of the cations from the calcium-free solution gives a further shift of — 35 mV. All shifts are explained quantitatively by assuming that changes in an external surface potential set up by fixed charges near the sodium channel produce the shifts. The model involves a diffuse double layer of counterions at the nerve surface and some binding of H+ ions and divalent ions to the fixed charges. Three types of surface groups are postulated: (1) an acid pKa = 2.88, charge density —0.9 nm-2; (2) an acid pKa = 4.58, charge density —0.58 nm-2; (3) a base pKa = 6.28, charge density + 0.33 nm-2. The two acid groups also bind Ca2+ ions with a dissociation constant K = 28 M. Reasonable agreement can also be obtained with a lower net surface charge density and stronger binding of divalent ions and H+ ions.