Cited by 38Proteoglycans and glycosaminoglycans researchIon channel regulation and functionNeuroscience and Neuropharmacology Research

Chondroitin Sulfates Act as Extracellular Gating Modifiers on Voltage-Dependent Ion Channels

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Vigetti, Davide, Andrini, Olga, Clerici, Moira, Negrini, Daniela, Passi, Alberto, Moriondo, Andrea·2008·Cellular Physiology and Biochemistry

Abstract

BACKGROUND/AIMS: Chondroitin sulfates are glycosaminoglycans bound to core proteins of proteoglycans in the extracellular matrix and perineuronal nets surrounding many types of neurones. Chondroitin 4- and chondroitin 6- sulfate can bind calcium ions with different affinities, depending on their sulfation position. Extracellular calcium plays a key role in determining the transmembrane potential sensed by voltage-operated ion channels (VOCs) by means of the "surface screening effect" theory (Gouy-Chapman-Stern theory). We wanted to test whether chondroitin sulfates at physiological concentration can effectively modulate the gating properties of VOCs. METHODS: We recorded in patch-clamp experiments the shift of h and voltage-dependent calcium currents activation curves of Xenopus laevis photoreceptors perfused with chondroitin sulfate solutions in physiological extracellular calcium concentration. RESULTS: We found that chondroitin 4- and 6- sulfate, with different capabilities, can shift the activation curve of h and voltage-dependent calcium channels, compatibly with the surface screening effect theory. CONCLUSION: We conclude that chondroitin sulfates can alter VOCs gating by modulating the calcium concentration in the extracellular microenvironment. This phenomenon may explain why alterations in the chondroitin sulfation and abundance in the extracellular matrix are found along with altered neuronal function in pathological conditions.

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  1. Ames A, 3rd, Nesbett FB: Intracellu- lar and extracellular compartments of mammalian central nervous tis- sue. J Physiol (Lond) 1966;184:215- 238.
  2. Williams C, Villegas M, Atkinson R, Miller CA: Chondroitin sulfate proteoglycan specific to retinal horizon- tal neurons. J Comp Neurol 1998;390:268-277.
  3. Morgans CW, Ren G, Akileswaran L: Lo- calization of nyctalopin in the mamma- lian retina. Eur J Neurosci 2006;23:1163- 1171.
  4. Vigetti/Andrini/Clerici/Negrini/Passi/Moriondo Cell Physiol Biochem 2008;22:137-146

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Authors
Vigetti, D., Andrini, O., Clerici, M., Negrini, D., Passi, A., & Moriondo, A.
Affiliations
University of Insubria (IT)
Venue
Cellular Physiology and Biochemistry
Publisher
S. Karger AG
Published
2008
Volume
22
Issue
1-4
Pages
137-146
ISSN
  • 1015-8987 (ISSN-L)
  • 1421-9778
OpenAlex
W2013271982
Cited by
38
References
44
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 5 · 2022: 1 · 2021: 2 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 1 · 2012: 3
FWCI
0.44
Type
journal article
Language
en
Open access
Locations
  • Cellular Physiology and Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • IrInSubria (University of Insubria) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
chondroitin sulfate, chondroitin 4-sulfate, chondroitin-6-sulfate, voltage-gated ion channels, channel gating, Gouy-Chapman-Stern theory, extracellular calcium, H+ channel, voltage-gated calcium channels, photoreceptors, extracellular matrix, glycosaminoglycans, sulfation
MeSH terms
Animals, Calcium, Chondroitin Sulfates, Electrophoresis, Polyacrylamide Gel, Extracellular Space, Ion Channels, Xenopus, Calcium Channels, Ion Channel Gating

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