The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States
Abstract
Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the genetic identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. Phylogenetic analysis indicates that avian HEV is genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.
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Cites
A novel virus in swine is closely related to the human hepatitis E virus
Meng, X.-J., Purcell, R. H., Halbur, P. G., Lehman, J. R., Webb, D. M., Tsareva, T. S., Haynes, J. S., Thacker, B. J., & Emerson, S. U. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.18.9860 · Cited by 1,152
Self-citationHepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome
Tam, A. W., Smith, M. M., Guerra, M. E., Huang, C.-C., Bradley, D. W., Fry, K. E., & Reyes, G. R. (1991) · Virology · doi:10.1016/0042-6822(91)90760-9 · Cited by 1,065
Isolation of a cDNA from the Virus Responsible for Enterically Transmitted Non-A, Non-B Hepatitis
Reyes, G. R., Purdy, M. A., Kim, J., Luk, K.-C., Young, L. M., Fry, K. E., & Bradley, D. W. (1990) · Science · doi:10.1126/science.2107574 · Cited by 867
Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses
Kamer, G., & Argos, P. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7269 · Cited by 832
Genetic and Experimental Evidence for Cross-Species Infection by Swine Hepatitis E Virus
Meng, X.-J., Halbur, P. G., Shapiro, M. S., Govindarajan, S., Bruna, J. D., Mushahwar, I. K., Purcell, R. H., & Emerson, S. U. (1998) · Journal of Virology · doi:10.1128/jvi.72.12.9714-9721.1998 · Cited by 582
Self-citationKoonin, E. V., Gorbalenya, A. E., Purdy, M. A., Rozanov, M. N., Reyes, G. R., & Bradley, D. W. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.17.8259 · Cited by 530
Viral Hepatitis and Liver Disease
Gupta, S., & Ott, M. (1998) · Principles of Molecular Medicine · doi:10.1007/978-1-59259-726-0_43 · Cited by 518
Current topics in Microbiology and Immunology
Panouse, J. (1976) · Biochimie · doi:10.1016/s0300-9084(76)80211-8 · Cited by 435
Molecular cloning and sequencing of the mexico isolate of hepatitis E virus (HEV)
Huang, C.-C., Nguyen, D., Fernandez, J., Yun, K. Y., Fry, K. E., Bradley, D. W., Tam, A. W., & Reyes, G. R. (1992) · Virology · doi:10.1016/0042-6822(92)90230-m · Cited by 333
Halbur, P. G., Kasorndorkbua, C., Gilbert, C., Guenette, D., Potters, M. B., Purcell, R. H., Emerson, S. U., Toth, T. E., & Meng, X. J. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.3.918-923.2001 · Cited by 333
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Cited by
Emerson, S. U., & Purcell, R. H. (2003) · Reviews in Medical Virology · doi:10.1002/rmv.384 · Cited by 785
Hepatitis E Virus: Animal Reservoirs and Zoonotic Risk
Meng, X. J. (2010) · Veterinary Microbiology · doi:10.1016/j.vetmic.2009.03.017 · Cited by 491
Self-citationHepatitis-E-Virus: Stellungnahmen des Arbeitskreises Blut des Bundesministeriums für Gesundheit
Gesundheit, M. D. A. B. D. B. F. (2015) · Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz · doi:10.1007/s00103-014-2103-4 · Cited by 374
Zoonotic hepatitis E: animal reservoirs and emerging risks
Pavio, N., Meng, X.-J., & Renou, C. (2010) · Veterinary Research · doi:10.1051/vetres/2010018 · Cited by 344
Self-citationFeagins, A. R., Opriessnig, T., Guenette, D. K., Halbur, P. G., & Meng, X.-J. (2007) · Journal of General Virology · doi:10.1099/vir.0.82613-0 · Cited by 342
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Metadata
- Authors
- Haqshenas, G., Shivaprasad, H. L., Woolcock, P. R., Read, D. H., & Meng, X. J.
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 2001-10-01
- Volume
- 82
- Issue
- 10
- Pages
- 2449-2462
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2150068310
- Cited by
- 353
- References
- 32
- FWCI
- 18.48
- Type
- journal article
- Language
- en
- Open access
- closed
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- Haqshenas, G., Shivaprasad, H. L., Woolcock, P. R., Read, D. H., & Meng, X. J. (2001). Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States. Journal of General Virology. https://doi.org/10.1099/0022-1317-82-10-2449
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