The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans
Abstract
The green fluorescent protein (GFP) of Aequorea victoria has been developed here as a reporter for gene expression and protein localization in Candida albicans. When wild-type (wt) GFP was expressed in C. albicans, it was not possible to detect fluorescence or a translation product for the wt protein. Since this was probably due in part to the presence of the non-canonical CTG serine codon in the Aequorea sequence, this codon was changed to the leucine codon TTG. C. albicans cells expressing this construct contained GFP mRNA but were non-fluorescent and contained no detectable translation product. Hence a codon-optimized GFP gene was constructed in which all of the 239 amino acids are encoded by optimal codons for C albicans. In this gene were also incorporated two previously identified mutations in the chromophore that increase GFP fluorescence. C. albicans cells expressing this yeast-enhanced GFP gene (yEGFP3) are fluorescent and contain GFP protein. yEGFP3 can be used as a versatile reporter of gene expression in C. albicans and Saccharomyces cerevisiae and the optimized GFP described here should have broad applications in these and other fungal species.
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Cites
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Self-citationGuide to Yeast Genetics and Molecular Biology
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Cited by
Janke, C., Magiera, M. M., Rathfelder, N., Taxis, C., Reber, S., Maekawa, H., Moreno‐Borchart, A., Doenges, G., Schwob, E., Schiebel, E., et al. (2004) · Yeast · doi:10.1002/yea.1142 · Cited by 2,119
Localization of ASH1 mRNA Particles in Living Yeast
Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., & Long, R. M. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80143-4 · Cited by 1,622
Codon bias and heterologous protein expression
Gustafsson, C., Govindarajan, S., & Minshull, J. (2004) · Trends in Biotechnology · doi:10.1016/j.tibtech.2004.04.006 · Cited by 1,330
Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines
Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K., & Schiebel, E. (1999) · Yeast · doi:10.1002/(sici)1097-0061(199907)15:10b<963::aid-yea399>3.0.co;2-w · Cited by 1,047
Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R., & Piston, D. W. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78307-3 · Cited by 850
Metadata
- Authors
- Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A. R., Falkow, S., & Brown, A. J. P.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 1997-02-01
- Volume
- 143
- Issue
- 2
- Pages
- 303-311
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2130652548
- Cited by
- 592
- References
- 41
- FWCI
- 10.12
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A. R., Falkow, S., & Brown, A. J. P. (1997). Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology. https://doi.org/10.1099/00221287-143-2-303
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