The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Experimental reproducibility in flow-chamber biofilms
Abstract
The structural organization of microbial communities is influenced by many factors, e.g. nutrient composition, shear stress and temperature. This paper presents a general method for quantitative comparison of biofilm structures and assessment of experimental reproducibility between independent biofilm experiments. By using a novel computer program, COMSTAT, biofilm structures of Pseudomonas aeruginosa and an isogenic rpoS mutant were quantified. The strains were tagged with the green fluorescent protein (GFP) and grown in flow chambers with a defined minimal medium as substrate. Three independent rounds of biofilm experiments were performed and in each round, each of the two variants was grown in two separate channels. Nine image stacks were acquired in each channel 146 h after inoculation. An analysis of variance model incorporating the factors experiment round, bacterial strain, channel number and image stack number was used to analyse the data calculated by COMSTAT. Experimental reproducibility was verified by estimating the magnitude of the variance of the effects round (sigma(2)R) and the interaction between bacterial strain and round (sigma(2)BR). Mean thickness of the wild-type and rpoS mutant biofilms was estimated at 6.31 microm (SE 0.81 microm) and 16.85 microm (SE 0.87 microm), respectively.
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Quantification of biofilm structures by the novel computer program comstat
Heydorn, A., Nielsen, A. T., Hentzer, M., Sternberg, C., Givskov, M., Ersbøll, B. K., & Molin, S. (2000) · Microbiology · doi:10.1099/00221287-146-10-2395 · Cited by 2,179
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Psl trails guide exploration and microcolony formation in Pseudomonas aeruginosa biofilms
Zhao, K., Tseng, B. S., Beckerman, B., Jin, F., Gibiansky, M. L., Harrison, J. J., Luijten, E., Parsek, M. R., & Wong, G. C. L. (2013) · Nature · doi:10.1038/nature12155 · Cited by 401
Self-citationAntibiofilm Activity of an Exopolysaccharide from Marine Bacterium Vibrio sp. QY101
Jiang, P., Li, J., Han, F., Duan, G., Lu, X., Gu, Y., & Yu, W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018514 · Cited by 165
Devaraj, A., Buzzo, J. R., Mashburn-Warren, L., Gloag, E. S., Novotny, L. A., Stoodley, P., Bakaletz, L. O., & Goodman, S. D. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1909017116 · Cited by 133
Freire, M. O., Devaraj, A., Young, A., Navarro, J. B., Downey, J. S., Chen, C., Bakaletz, L. O., Zadeh, H. H., & Goodman, S. D. (2017) · Molecular Oral Microbiology · doi:10.1111/omi.12155 · Cited by 55
Metadata
- Authors
- Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., & Molin, S.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2000-10-01
- Volume
- 146
- Issue
- 10
- Pages
- 2409-2415
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2110017940
- Cited by
- 264
- References
- 36
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., & Molin, S. (2000). Experimental reproducibility in flow-chamber biofilms. Microbiology. https://doi.org/10.1099/00221287-146-10-2409
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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