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Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae.
Abstract
Cells of the yeast Saccharomyces cerevisiae IGC 4072 grown in medium with acetic acid produced a mediated transport system for acetic acid that behaved as an electroneutral proton symport for the anionic form of the acid. The system could transport propionate and formate but not lactate and pyruvate. Uptake of labelled lactic acid was negligible, no mediated transport system activity for this acid being found. The acetate transporter was also found in cells grown in lactic acid or ethanol media, suggesting that the carrier did not require the presence of an external inducer. When cells were grown in lactic acid medium, uptake of labelled acetic acid, at pH 5.0, was biphasic and consistent with the presence of two distinct transport modes for the acid. One of these components corresponded to the acetate/proton symport, and the higher affinity system corresponded to a more general monocarboxylate carrier that could also transport lactate, pyruvate and propionate. Both systems were subject to repression by glucose, fructose, sucrose, maltose or galactose. In glucose-repressed cells, the undissociated form of the acids appeared to be the only one that could cross the plasma membrane, a diffusion mechanism being involved in the acid uptake. Under these growth conditions and when the extracellular pH was lower than that of the cytosol, accumulation of the acid could also be observed, it being a function of the delta pH.
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“In S. cerevisiae, ADY2 (also called ATO1) encodes a permease-mediating uptake of acetate–propionate–formate present in ethanol or acetate-grown cells (Casal et al., 1996; Paiva et al., 2004; Pacheco et al., 2012).”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Casal et al., 1996
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Metadata
- Authors
- Casal, M., Cardoso, H., & Leao, C.
- Affiliations
- University of Minho (PT)
- Department of Biology, University of Minho, 4719 Braga Codex, Portugal
- ORCID
- Casal, Margarida
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 1996-06-01
- Volume
- 142
- Issue
- 6
- Pages
- 1385-1390
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2115422703
- Cited by
- 200
- References
- 17
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 5 · 2023: 6 · 2022: 10 · 2021: 14 · 2020: 9 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 8 · 2015: 11 · 2014: 6 · 2013: 8 · 2012: 10
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Johns Hopkins University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- Saccharomyces cerevisiae, proton symport, glucose repression, lactate utilization, pyruvate uptake, pH gradient, acid accumulation
- MeSH terms
- Acetates, Biological Transport, Carbohydrates, Carrier Proteins, Diffusion, Formates, Fungal Proteins, Lactates, Propionates, Protons, Pyruvates, Saccharomyces cerevisiae, Pyruvic Acid, Acetic Acid, Lactic Acid, Monocarboxylic Acid Transporters
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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