Capnocytophaga gingivalis: effects of glucose concentration on growth and hydrolytic enzyme production
Abstract
In chemostat culture, the microaerophilic, CO2 requiring, gingival-plaque-associated bacterium Capnocytophaga gingivalis responded to the addition of glucose (1-6 g I-1) by doubling its growth rate and increasing its biomass yield fivefold. The data suggest that the glucose is catabolized by a fully aerobic route. Rather than repressing hydrolytic enzymes which might be associated with pathogenic properties, glucose enhanced the specific activity of aminopeptidase, trypsin-like protease, acid and alkaline phosphatase and alpha-glucosidase in comparison with a control culture grown in a tryptone/thiamin medium. Thus, the supply of glucose could be of importance in maximizing the pathogenic potential of this organism.
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Capnocytophaga species are saccharolytic organisms that have been shown to demonstrate increased proteolytic potential in the presence of elevated glucose levels.
“Capnocytophaga species are saccharolytic organisms that have been shown to demonstrate increased proteolytic potential in the presence of elevated glucose levels.220”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [220]
Fusobacterium nucleatum and A actinomycetemcomitans are well recognized in periodontal disease, and F nucleatum is noted for its role in the development of anaerobic microenvironments that facilitate the outgrowth of anaerobic periodontal pathogens.
“Fusobacterium nucleatum and A actinomycetemcomitans are well recognized in periodontal disease,220, 221 and F nucleatum is noted for its role in the development of anaerobic microenvironments that facilitate the outgrowth of anaerobic periodontal pathogens.222”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [220]
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- Authors
- Spratt, D. A., Greenman, J., & Schaffer, A. G.
- Affiliations
- University of the West of England (GB)
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 1996-08-01
- Volume
- 142
- Issue
- 8
- Pages
- 2161-2164
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2010048148
- Cited by
- 24
- References
- 11
- Citations by year
- 2025: 2 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2017: 1 · 2016: 2 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Capnocytophaga gingivalis, glucose concentration, bacterial growth, hydrolytic enzymes, aminopeptidase, trypsin-like protease, phosphatase, alpha-glucosidase, chemostat culture, glucose catabolism, pathogenicity, microaerophilic bacteria
- MeSH terms
- Acid Phosphatase, Alkaline Phosphatase, alpha-Glucosidases, Aminopeptidases, Capnocytophaga, Culture Media, Dental Plaque, Gingiva, Glucose, Humans, Hydrolases, Serine Endopeptidases, Biomass
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