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Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa
Abstract
Pseudomonas aeruginosa produces multiple virulence factors and causes different types of infections. Previous clinical studies identified P. aeruginosa isolates that lack individual virulence factors. However, the impact of losing several virulence factors simultaneously on the in vivo virulence of P. aeruginosa is not completely understood. The P. aeruginosa cell-to-cell communication system, or quorum sensing (QS), controls the production of several virulence factors. Animal studies using constructed QS mutants indicated that loss of the QS system severely impacts the virulence of P. aeruginosa. In this study, we tried to determine if deficiency within the QS system compromises the ability of P. aeruginosa to establish infections in humans. We have identified five QS-deficient strains through screening 200 isolates from patients with urinary tract, lower respiratory tract and wound infections. These strains lacked LasB and LasA activities and produced either no or very low levels of the autoinducers N-(3-oxododecanoyl) homoserine lactone and N-butyryl homoserine lactone. PCR analysis revealed that three isolates contained all four QS genes (lasI, lasR, rhlI and rhlR) while two isolates lacked both the lasR and rhlR genes. We also examined the five isolates for other virulence factors. The isolates produced variable levels of exotoxin A and, with one exception, were deficient in pyocyanin production. One isolate produced the type III secretion system (TTSS) effector proteins ExoS and ExoT, two isolates produced ExoT only and two isolates produced no TTSS proteins. The isolates produced weak to moderate biofilms on abiotic surfaces. Analysis of the patients' data revealed that two of the isolates represented a single strain that was isolated twice from the same patient within a 1 month interval. One QS-deficient clinical isolate (CI-1) lacked all tested virulence factors and produced a weak biofilm. These results suggest that naturally occurring QS-deficient strains of P. aeruginosa do occur and are capable of causing infections; and, that besides the known virulence factors, additional factors may contribute to the ability of certain strains such as CI-1 to establish an infection.
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“Our study has also shown the lack of Pseudomonas virulence factors and genes for QS molecules within wound isolates, which goes in line with previous studies [64] confirming that spontaneous QS- and virulence-deficient P. aeruginosa mutants are still capable of causing wound infections.”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [64]
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Metadata
- Authors
- Schaber, J. A., Carty, N. L., McDonald, N. A., Graham, E. D., Cheluvappa, R., Griswold, J. A., & Hamood, A. N.
- Affiliations
- Texas Tech University (US)
- Venue
- Journal of Medical Microbiology
- Publisher
- Microbiology Society
- Published
- 2004-09-01
- Volume
- 53
- Issue
- 9
- Pages
- 841-853
- ISSN
- 0022-2615 (ISSN-L)
- 1473-5644
- OpenAlex
- W2163902276
- Cited by
- 224
- References
- 66
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 10 · 2021: 11 · 2020: 7 · 2019: 10 · 2018: 9 · 2017: 5 · 2016: 13 · 2015: 15 · 2014: 15 · 2013: 16 · 2012: 18
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Texas Tech University
- Directorate for Biological Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
- Keywords
- Pseudomonas aeruginosa, quorum sensing, virulence factors, clinical isolates, lasB, LASA, autoinducers, LasR, RhlR, type III secretion system, exoS, ExoT, pyocyanin, exotoxin A, biofilm formation, urinary tract infection, lower respiratory tract infection, wound infection
- MeSH terms
- Bacterial Proteins, Humans, Metalloendopeptidases, Mutation, Pseudomonas aeruginosa, Pseudomonas Infections, Transcription, Genetic, Virulence, 4-Butyrolactone, Gene Expression Regulation, Bacterial, Biofilms
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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