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Cited by 224Bacterial biofilms and quorum sensingEscherichia coli research studiesVibrio bacteria research studies

The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.

Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa

Schaber, J. Andy, Carty, Nancy L., McDonald, Naomi A., Graham, Eric D., Cheluvappa, Rajkumar, Griswold, John A., Hamood, Abdul N.·2004·Journal of Medical Microbiology
DOI 10.1099/jmm.0.45617-0
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Abstract

Pseudomonas aeruginosa produces multiple virulence factors and causes different types of infections. Previous clinical studies identified P. aeruginosa isolates that lack individual virulence factors. However, the impact of losing several virulence factors simultaneously on the in vivo virulence of P. aeruginosa is not completely understood. The P. aeruginosa cell-to-cell communication system, or quorum sensing (QS), controls the production of several virulence factors. Animal studies using constructed QS mutants indicated that loss of the QS system severely impacts the virulence of P. aeruginosa. In this study, we tried to determine if deficiency within the QS system compromises the ability of P. aeruginosa to establish infections in humans. We have identified five QS-deficient strains through screening 200 isolates from patients with urinary tract, lower respiratory tract and wound infections. These strains lacked LasB and LasA activities and produced either no or very low levels of the autoinducers N-(3-oxododecanoyl) homoserine lactone and N-butyryl homoserine lactone. PCR analysis revealed that three isolates contained all four QS genes (lasI, lasR, rhlI and rhlR) while two isolates lacked both the lasR and rhlR genes. We also examined the five isolates for other virulence factors. The isolates produced variable levels of exotoxin A and, with one exception, were deficient in pyocyanin production. One isolate produced the type III secretion system (TTSS) effector proteins ExoS and ExoT, two isolates produced ExoT only and two isolates produced no TTSS proteins. The isolates produced weak to moderate biofilms on abiotic surfaces. Analysis of the patients' data revealed that two of the isolates represented a single strain that was isolated twice from the same patient within a 1 month interval. One QS-deficient clinical isolate (CI-1) lacked all tested virulence factors and produced a weak biofilm. These results suggest that naturally occurring QS-deficient strains of P. aeruginosa do occur and are capable of causing infections; and, that besides the known virulence factors, additional factors may contribute to the ability of certain strains such as CI-1 to establish an infection.

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Crossref · 10.1099/jmm.0.45617-0

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  1. Griswold, 2001, Length of antibiotic treatment influences risk of repeated Pseudomonas aeruginosa infections, Curr Opin Clin Exp Res, 3, 186

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Metadata

Authors
Schaber, J. A., Carty, N. L., McDonald, N. A., Graham, E. D., Cheluvappa, R., Griswold, J. A., & Hamood, A. N.
Affiliations
Texas Tech University (US)
ORCID
Venue
Journal of Medical Microbiology
Publisher
Microbiology Society
Published
2004-09-01
Volume
53
Issue
9
Pages
841-853
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
DOI
10.1099/jmm.0.45617-0
OpenAlex
W2163902276
Cited by
224
References
66
Citations by year
2026: 6 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 10 · 2021: 11 · 2020: 7 · 2019: 10 · 2018: 9 · 2017: 5 · 2016: 13 · 2015: 15 · 2014: 15 · 2013: 16 · 2012: 18
FWCI
2.72
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • Texas Tech University
  • Directorate for Biological Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
Keywords
Pseudomonas aeruginosa, quorum sensing, virulence factors, clinical isolates, lasB, LASA, autoinducers, LasR, RhlR, type III secretion system, exoS, ExoT, pyocyanin, exotoxin A, biofilm formation, urinary tract infection, lower respiratory tract infection, wound infection
MeSH terms
Bacterial Proteins, Humans, Metalloendopeptidases, Mutation, Pseudomonas aeruginosa, Pseudomonas Infections, Transcription, Genetic, Virulence, 4-Butyrolactone, Gene Expression Regulation, Bacterial, Biofilms

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