Cited by 91Respiratory viral infections researchMicrobial infections and disease researchPneumonia and Respiratory Infections

Nontypeable Haemophilus influenzae and Streptococcus pneumoniae bind respiratory syncytial virus glycoprotein

Avadhanula, Vasanthi, Wang, Yan, Portner, Allen, Adderson, Elisabeth·2007·Journal of Medical Microbiology

Abstract

Respiratory syncytial virus (RSV) infection is associated with secondary bacterial infections caused by nontypeable Haemophilus influenzae (NTHi) and Streptococcus pneumoniae. The pathogenesis of these complications is not completely understood; however, viral infection of respiratory epithelial cells promotes colonization by these bacteria. In the present study, RSV virions associated with NTHi and pneumococci in an inoculum-dependent manner in a fluid-phase binding assay. Adherence of NTHi and S. pneumoniae to epithelial cells transiently expressing RSV G glycoprotein was 2- and 2.2-fold higher, respectively, than adhesion to cells transfected with the vector alone (P <0.01). Furthermore, 4.6- and 6.2-fold larger numbers of NTHi and pneumococci bound to cells expressing a membrane-bound full-length RSV G protein than to cells expressing a truncated non-membrane-bound protein (P ≤0.005). Pre-incubating cells expressing membrane-bound G protein with blocking anti-RSV G antibodies reduced bacterial adherence by 78–84 % (P ≤0.005). These studies demonstrate that RSV G protein is a receptor for both NTHi and S. pneumoniae. Strategies to prevent this interaction may reduce the incidence of secondary bacterial complications of RSV infection.

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Crossref · 10.1099/jmm.0.47086-0

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  • RSV induces Pseudomonas aeruginosa, S. pneumoniae, and H. influenzae adherence to airway epithelial cells.

    “RSV was shown to induce Pseudomonas aeruginosa, S. pneumoniae, and H. influenzae adherence to airway epithelial cells (12, 70, 95, 292)”

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Authors
Avadhanula, V., Wang, Y., Portner, A., & Adderson, E.
Affiliations
St. Jude Children's Research Hospital (US)
Venue
Journal of Medical Microbiology
Published
2007-09-01
Volume
56
Issue
9
Pages
1133-1137
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
OpenAlex
W2136782345
Cited by
91
References
14
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 12 · 2019: 8 · 2018: 1 · 2017: 9 · 2016: 5 · 2015: 7 · 2014: 5 · 2013: 4 · 2012: 4
FWCI
2.07
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Lebanese Syrian Associated Charities
  • National Institutes of Health
  • National Cancer Institute (CA21765, P30CA021765)
Topics
  • Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
Keywords
respiratory syncytial virus, nontypeable Haemophilus influenzae, Streptococcus pneumoniae, bacterial adhesion, viral-bacterial interactions, secondary bacterial infection, bacterial receptors, membrane glycoproteins, airway epithelial cells
MeSH terms
Animals, Bacterial Adhesion, Cricetulus, Epithelial Cells, Haemophilus Infections, Haemophilus influenzae, Cricetinae, Pneumococcal Infections, Protein Binding, Streptococcus pneumoniae, Viral Fusion Proteins, CHO Cells, Respiratory Syncytial Virus Infections

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