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Cited by 25Probiotics and Fermented FoodsBacterial Genetics and BiotechnologyReproductive tract infections research

Identification of lactose phosphotransferase systems in Lactobacillus gasseri ATCC 33323 required for lactose utilization

Francl, Alyssa L., Hoeflinger, Jennifer L., Miller, Michael J.·2012·Microbiology
DOI 10.1099/mic.0.052928-0
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Abstract

Improving the annotation of sugar catabolism-related genes requires functional characterization. Our objective was to identify the genes necessary for lactose utilization by Lactobacillus gasseri ATCC 33323 (NCK334). The mechanism of lactose transport in many lactobacilli is a lactose/galactose-specific permease, yet no orthologue was found in NCK334. Characterization of an EI knockout strain [EI (enzyme I) is required for phosphotransferase system transporter (PTS) function] demonstrated that L. gasseri requires PTS(s) to utilize lactose. In order to determine which PTS(s) were necessary for lactose utilization, we compared transcript expression profiles in response to lactose for the 15 complete PTSs identified in the NCK334 genome. PTS 6CB (LGAS_343) and PTS 8C (LGAS_497) were induced in the presence of lactose 107- and 53-fold, respectively. However, L. gasseri ATCC 33323 PTS 6CB, PTS 8C had a growth rate similar to that of the wild-type on semisynthetic deMan, Rogosa, Sharpe (MRS) medium with lactose. Expression profiles of L. gasseri ATCC 33323 PTS 6CB, PTS 8C in response to lactose identified PTS 9BC (LGAS_501) as 373-fold induced, whereas PTS 9BC was not induced in NCK334. Elimination of growth on lactose required the inactivation of both PTS 6CB and PTS 9BC. Among the six candidate phospho-β-galactosidase genes present in the NCK334 genome, LGAS_344 was found to be induced 156-fold in the presence of lactose. In conclusion, we have determined that: (1) NCK334 uses a PTS to import lactose; (2) PTS 6CB and PTS 8C gene expression is strongly induced by lactose; and (3) elimination of PTS 6CB and PTS 9BC is required to prevent growth on lactose.

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  • 25 unique COGs are implicated in the import and processing of human milk sugars.

    “Specifically, 25 unique COGs are implicated in the import and processing of human milk sugars [84] , while others are associated with host-immune interactions [85] and osmotic and pH regulation [86] , i.e., functions that support survival in the infant gut [23,87,88] .”

    From Genome-scale insights into the metabolic landscape and evolutionary development of Bifidobacterium bifidum (2026) · cites this paper as [84]

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Authors
Francl, A. L., Hoeflinger, J. L., & Miller, M. J.
Affiliations
University of Illinois Urbana-Champaign (US)
ORCID
Venue
Microbiology
Publisher
Microbiology Society
Published
2012-04-01
Volume
158
Issue
4
Pages
944-952
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
DOI
10.1099/mic.0.052928-0
OpenAlex
W2027992722
Cited by
25
References
37
Citations by year
2026: 1 · 2024: 1 · 2023: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 2 · 2012: 1
FWCI
2.95
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. Department of Agriculture
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
Lactobacillus gasseri, lactose utilization, phosphotransferase system, lactose transport, gene expression regulation, sugar catabolism, transcriptome
MeSH terms
Bacterial Proteins, Culture Media, Genes, Bacterial, Lactobacillus, Lactose, Phosphoenolpyruvate Sugar Phosphotransferase System, Gene Expression Regulation, Bacterial, Computational Biology, Gene Knockout Techniques, Transcriptome

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