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UCHIME2: improved chimera prediction for amplicon sequencing

Open access
Edgar, Robert C.·2016·bioRxiv (Cold Spring Harbor Laboratory)

Abstract

Amplicon sequencing generates chimeric reads which can cause spurious inferences of biological variation. I describe UCHIME2, an update of the popular UCHIME chimera detection algorithm with new modes optimized for high-resolution biological sequence reconstruction (“denoising”) and other applications. I show that chimera frequency correlates inversely with divergence, that error-free chimera prediction from sequence is impossible in principle, and that UCHIME2 achieves higher detection accuracy than previous methods.

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Metadata

Authors
Edgar, R. C.
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2016-09-12
OpenAlex
W2520140228
Cited by
351
References
4
Type
posted content
Language
en
Open access
green

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CoChat. (2026). Citation check: UCHIME2: improved chimera prediction for amplicon sequencing. https://app.cochat.ai/papers/10.1101/074252
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Edgar, R. C. (2016). UCHIME2: improved chimera prediction for amplicon sequencing. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/074252

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