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A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
Open accessPreprint — published as 10.15252/embj.2021110352 · Paper page
Abstract
Beyond its role in cellular homeostasis, autophagy plays anti- and pro-microbial roles in host-microbe interactions, both in animals and plants. One prominent role of anti-microbial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in depletion of a bacterial virulence factor and unravels an unprecedented pathogen strategy to counteract defense-related autophagy.
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Metadata
- Authors
- Leong, J. X., Raffeiner, M., Spinti, D., Langin, G., Franz-Wachtel, M., Guzman, A. R., Kim, J.-G., Pandey, P., Minina, A. E., Macek, B., Hafrén, A., Bozkurt, T. O., Mudgett, M. B., Börnke, F., Hofius, D., & Üstün, S.
- Affiliations
- University of Tübingen (DE)
- Leibniz Institute of Vegetable and Ornamental Crops (DE)
- Stanford University (US)
- Imperial College London (GB)
- Swedish University of Agricultural Sciences (SE)
- University of Potsdam (DE)
- ORCID
- Venue
- The EMBO Journal
- Publisher
- openRxiv
- Published
- 2021-03-17
- Volume
- 41
- Issue
- 13
- Pages
- e110352-e110352
- ISSN
- 0261-4189 (ISSN-L)
- PMC
- PMC9251887
- OpenAlex
- W4281979571
- Cited by
- 89
- References
- 1
- Citations by year
- 2026: 15 · 2025: 17 · 2024: 19 · 2023: 25 · 2022: 10 · 2021: 3
- FWCI
- 9.77
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Epsilon Open Archive (Sveriges lantbruksuniversitet biblioteket (Swedish University of Agricultural Sciences)) (acceptedVersion)
- publish.UP (University of Potsdam) (submittedVersion, cc-by)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 1101, UE188/2‐1, BO1961/5‐2)
- Svenska Forskningsrådet Formas (2017-01596, 2016-01044)
- Vetenskapsrådet (2020-05327, 2016-04562)
- Biotechnology and Biological Sciences Research Council (BB/T006102/1)
- Division of Integrative Organismal Systems (2026368)
- HORIZON EUROPE Marie Sklodowska-Curie Actions (799433)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- autophagy, xenophagy, plant-bacteria interactions, Xanthomonas campestris pv. vesicatoria, type III effectors, E3 ubiquitin ligase, NBR1, selective autophagy, plant defense
- MeSH terms
- Animals, Autophagy, Bacteria, Plant Diseases, Virulence Factors, Host-Pathogen Interactions
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