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PreprintCited by 99Silk-based biomaterials and applicationsSpider Taxonomy and Behavior StudiesSilkworms and Sericulture Research

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Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

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Kono, Nobuaki, Nakamura, Hiroyuki, Mori, Masaru, Yoshida, Yuki, Ohtoshi, Rintaro, Malay, Ali D, Pedrazzoli Moran, Daniel A, Tomita, Masaru, Numata, Keiji, Arakawa, Kazuharu·2021·Proceedings of the National Academy of Sciences
DOI 10.1101/2021.04.22.441049PMC PMC8346794

Preprint — published as 10.1073/pnas.2107065118 · Paper page

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Abstract

Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach combining high-quality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomics of four Nephilinae spiders. We observed the consistent presence of the MaSp3B spidroin unique to this subfamily, as well as several non-spidroin SpiCE proteins. Artificial synthesis and combination of these components in vitro showed that the multicomponent nature of dragline silk, including MaSp3B and SpiCE, along with MaSp1 and MaSp2, is essential to realize the mechanical properties of spider dragline silk.

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    “The abundance ratios of the proteins were then quantified by intensity-based absolute quantification (iBAQ) (40) with shotgun proteomics of selectively reeled dragline silks (SI Appendix, Fig.”
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Metadata

Authors
Kono, N., Nakamura, H., Mori, M., Yoshida, Y., Ohtoshi, R., Malay, A. D., Pedrazzoli Moran, D. A., Tomita, M., Numata, K., & Arakawa, K.
Affiliations
  • Keio University (JP)
  • Spiber (Japan) (JP)
  • RIKEN Center for Sustainable Resource Science (JP)
  • Kyoto University (JP)
Corresponding author
Arakawa, Kazuharu
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
openRxiv
Published
2021-04-23
Volume
118
Issue
31
ISSN
0027-8424 (ISSN-L)
DOI
10.1101/2021.04.22.441049
PMC
PMC8346794
OpenAlex
W3183671013
Cited by
99
References
1
Citations by year
2026: 12 · 2025: 19 · 2024: 22 · 2023: 22 · 2022: 20 · 2021: 4
FWCI
4.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Sumitomo Foundation (190426)
  • Uehara Memorial Foundation (202010238)
  • Nakatsuji Foresight Foundation (NFF2020)
  • Ministry of Education, Culture, Sports, Science and Technology (21H02210)
  • Cabinet Office, Government of Japan
  • Ministry of Environment
  • Council for Science, Technology and Innovation
  • Japan Society for the Promotion of Science (190426, 21H02210, 18J21155, 23K21203)
  • Ministry of Environment and Sustainable Development
Topics
  • Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Spider Taxonomy and Behavior Studies
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Silkworms and Sericulture Research
Keywords
spider dragline silk, spidroin, silk mechanical properties, toughness, tensile strength, extensibility, biopolymers
MeSH terms
Animals, Fibroins, Spiders, Genome, Silk, Transcriptome

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