PreprintCited by 130Single-cell and spatial transcriptomicsGene expression and cancer classificationAI in cancer detection

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Leveraging information in spatial transcriptomics to predict super-resolution gene expression from histology images in tumors

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Pang, Minxing, Su, Kenong, Li, Mingyao·2021·bioRxiv (Cold Spring Harbor Laboratory)

DOI 10.1101/2021.11.28.470212

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Abstract

Recent developments in spatial transcriptomics (ST) technologies have enabled the profiling of transcriptome-wide gene expression while retaining the location information of measured genes within tissues. Moreover, the corresponding high-resolution hematoxylin and eosin-stained histology images are readily available for the ST tissue sections. Since histology images are easy to obtain, it is desirable to leverage information learned from ST to predict gene expression for tissue sections where only histology images are available. Here we present HisToGene, a deep learning model for gene expression prediction from histology images. To account for the spatial dependency of measured spots, HisToGene adopts Vision Transformer, a state-of-the-art method for image recognition. The well-trained HisToGene model can also predict super-resolution gene expression. Through evaluations on 32 HER2+ breast cancer samples with 9,612 spots and 785 genes, we show that HisToGene accurately predicts gene expression and outperforms ST-Net both in gene expression prediction and clustering tissue regions using the predicted expression. We further show that the predicted super-resolution gene expression also leads to higher clustering accuracy than observed gene expression. Gene expression predicted from HisToGene enables researchers to generate virtual transcriptomics data at scale and can help elucidate the molecular signatures of tissues.

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Authors: Pang, M., Su, K., & Li, M.
Affiliations: King Abdullah University of Science and Technology (SA)
University of Pennsylvania (US)
Corresponding author: Su, Kenong
Li, Mingyao
ORCID: Pang, Minxing
Li, Mingyao
Venue: bioRxiv (Cold Spring Harbor Laboratory)
Publisher: openRxiv
Published: 2021-11-28
DOI: 10.1101/2021.11.28.470212
OpenAlex: W3216067893
Cited by: 130
References: 37
Citations by year: 2026: 35 · 2025: 49 · 2024: 28 · 2023: 13 · 2022: 5
Type: posted content
Language: en
Open access: green
doi.org
Licence: cc-by-nc-nd
Locations: bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
Physical Sciences › Computer Science › Artificial Intelligence › AI in cancer detection
Keywords: spatial transcriptomics, HER2-positive breast cancer, vision transformer, tumor molecular signatures

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Leveraging information in spatial transcriptomics to predict super-resolution gene expression from histology images in tumors

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