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PreprintCited by 36Plant-Microbe Interactions and ImmunityImmune Response and InflammationPlant Pathogenic Bacteria Studies

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A wheat resistosome defines common principles of immune receptor channels

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Förderer, Alexander, Li, Ertong, Lawson, Aaron, Deng, Ya-nan, Sun, Yue, Logemann, Elke, Zhang, Xiaoxiao, Wen, Jie, Han, Zhifu, Chang, Junbiao, Chen, Yuhang, Schulze-Lefert, Paul, Chai, Jijie·2022·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2022.03.23.485489

Preprint — published as 10.1038/s41586-022-05231-w · Paper page

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Abstract

Plant intracellular nucleotide-binding leucine-rich repeat (NLRs) receptors detect pathogen effectors to trigger immune responses. Indirect recognition of a pathogen effector by the dicotyledonous Arabidopsis thaliana coiled-coil (CC) domain containing NLR (CNL) ZAR1 induces the formation of a large hetero-oligomeric protein complex, termed the ZAR1 resistosome, which functions as a calcium channel required for ZAR1-mediated immunity ( 1 – 3 ). Whether the resistosome and channel activities are conserved among plant CNLs remains unknown. We report here a cryogenic electron microscopy (cryo-EM) structure of the wheat CNL Sr35 in complex with the effector AvrSr35 of the wheat stem rust pathogen at 3.0 Å resolution. Direct effector binding to the leucine-rich repeats (LRRs) of Sr35 results in the formation of a pentameric Sr35-AvrSr35 complex, which we designate the Sr35 resistosome. Wheat Sr35 and Arabidopsis ZAR1 resistosomes bear striking structural similarity, including a previously unnoticed arginine cluster in the LRR domain that co-occurs and forms intramolecular interactions with the ‘EDVID’ motif in the CC domain. Electrophysiological measurements show that the Sr35 resistosome exhibits non-selective cation channel activity. These structural insights allowed us to generate novel variants of closely related wheat and barley orphan NLRs that recognize AvrSr35. Our data support the evolutionary conservation of CNL resistosomes in plants and demonstrate proof of principle for structure-based engineering of NLRs for crop improvement.

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Metadata

Authors
Förderer, A., Li, E., Lawson, A., Deng, Y.-N., Sun, Y., Logemann, E., Zhang, X., Wen, J., Han, Z., Chang, J., Chen, Y., Schulze-Lefert, P., & Chai, J.
Affiliations
  • University of Cologne (DE)
  • Max Planck Institute for Plant Breeding Research (DE)
  • Center for Life Sciences (CN)
  • Zhengzhou University (CN)
  • Chinese Academy of Sciences (CN)
Corresponding author
  • Chen, Yuhang
  • Chai, Jijie
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2022-03-23
DOI
10.1101/2022.03.23.485489
OpenAlex
W4220704509
Cited by
36
References
1
Citations by year
2026: 1 · 2025: 11 · 2024: 8 · 2023: 7 · 2022: 9
Type
posted content
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • Alexander von Humboldt-Stiftung (390686111)
  • European Molecular Biology Laboratory
  • Deutsche Forschungsgemeinschaft (EXC-2048, EXC-2048/1, 414786233, 390686111, 2048/1, SFB-1403-414 786 233)
  • Chinese Academy of Sciences
  • Max-Planck-Gesellschaft
  • Zhengzhou University
  • National Key Research and Development Program of China (2021YFA1300701, 2020YFA0509903)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
Keywords
resistosome, nucleotide-binding leucine-rich repeat receptors, coiled-coil domain, ZAR1, wheat stem rust, plant innate immunity, calcium channels, effector recognition, arginine cluster, barley, crop improvement

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