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PreprintCited by 27Bacterial biofilms and quorum sensingBiochemical and Structural CharacterizationAdvanced Electron Microscopy Techniques and Applications

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Architecture of the biofilm-associated archaic CupE pilus from Pseudomonas aeruginosa

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Böhning, Jan, Dobbelstein, Adrian, Sulkowski, Nina, Eilers, Kira, von Kügelgen, Andriko, Tarafder, Abul K., Alva, Vikram, Filloux, Alain, Bharat, Tanmay A. M.·2022·PLoS Pathogens
DOI 10.1101/2022.04.14.488289PMC PMC10104325

Preprint — published as 10.1371/journal.ppat.1011177

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Abstract

Chaperone-Usher Pathway (CUP) pili are major adhesins in Gram-negative bacteria, mediating bacterial adherence to biotic and abiotic surfaces. While classical CUP pili have been extensively characterized, little is known about so-called archaic CUP pili, which are phylogenetically widespread and promote biofilm formation by several human pathogens. In this study, we present the electron cryomicroscopy structure of the archaic CupE pilus from the opportunistic human pathogen Pseudomonas aeruginosa . We show that CupE pili consist of CupE1 subunits arranged in a zigzag architecture, with an N-terminal donor β-strand extending from each subunit into the next, where it is anchored by hydrophobic interactions, resulting in an overall flexible pilus arrangement. Imaging CupE pili on the surface of P. aeruginosa cells using electron cryotomography shows that CupE pili adopt variable curvatures in response to their environment, which may facilitate their role in promoting cohesion between bacterial cells. Finally, bioinformatic analysis shows the widespread abundance of cupE genes in isolates of P. aeruginosa and the co-occurrence of cupE with other cup clusters, suggesting interdependence of cup pili in regulating bacterial adherence within biofilms. Taken together, our study provides insights into the architecture of archaic CUP pili and their role in promoting cellular adhesion and biofilm formation in P. aeruginosa .

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Metadata

Authors
Böhning, J., Dobbelstein, A., Sulkowski, N., Eilers, K., von Kügelgen, A., Tarafder, A. K., Alva, V., Filloux, A., & Bharat, T. A. M.
Affiliations
  • University of Oxford (GB)
  • Max Planck Institute for Biology (DE)
  • Imperial College London (GB)
  • MRC Laboratory of Molecular Biology (GB)
Corresponding author
Bharat, Tanmay A. M.
ORCID
Venue
PLoS Pathogens
Publisher
openRxiv
Published
2022-04-14
Volume
19
Issue
4
Pages
e1011177-e1011177
ISSN
1553-7366 (ISSN-L)
DOI
10.1101/2022.04.14.488289
PMC
PMC10104325
OpenAlex
W4365483310
Cited by
27
References
1
Citations by year
2026: 12 · 2025: 5 · 2024: 8 · 2023: 1 · 2022: 1
FWCI
2.31
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • PLoS Pathogens (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Apollo (University of Cambridge) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Spiral (Imperial College London) (submittedVersion, other-oa)
  • Apollo
  • The Francis Crick Institute (cc-by)
Funders
  • Vallee Foundation
  • Wellcome Trust (202231, MR/N013468/1, 202231/Z/16/Z)
  • Francis Crick Institute (CC1063)
  • UK Research and Innovation (MC_UP_1201/31)
  • Leverhulme Trust
  • Lister Institute of Preventive Medicine
  • Medical Research Council (MR/N013468/1, MR/K501256/, MC_UP_1201/31, MR/N013468/, MR/K501256/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
Keywords
chaperone-usher pathway, CUPE, Pseudomonas aeruginosa, biofilm formation, bacterial adhesion, cryo-electron tomography, intersubunit interactions
MeSH terms
Humans, Fimbriae, Bacterial, Pseudomonas aeruginosa, Biofilms, Adhesins, Bacterial, Molecular Chaperones, Fimbriae Proteins

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