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PreprintCited by 65Plant Molecular Biology ResearchFlowering Plant Growth and CultivationLight effects on plants

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The F-box cofactor UFO redirects the LEAFY floral regulator to novel cis -elements

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Rieu, Philippe, Turchi, Laura, Thévenon, Emmanuel, Zarkadas, Eleftherios, Nanao, Max, Chahtane, Hicham, Tichtinsky, Gabrielle, Lucas, Jérémy, Blanc-Mathieu, Romain, Zubieta, Chloe, Schoehn, Guy, Parcy, François·2022·Nature Plants
DOI 10.1101/2022.06.14.495942

Preprint — published as 10.1038/s41477-022-01336-2

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Abstract

SUMMARY: In angiosperms, flower patterning requires the localized expression of the APETALA3 ( AP3 ) floral homeotic gene involved in petal and stamen development. AP3 is synergistically induced by the master transcription factor (TF) LEAFY (LFY) and the F-box protein UNUSUAL FLORAL ORGANS (UFO), but the molecular mechanism underlying this synergy has remained unknown. Here we show that the connection to ubiquitination pathways suggested by the F-box domain of UFO is mostly dispensable for its function and that UFO instead acts by forming a transcriptional complex with LFY and binds to newly discovered regulatory elements. Cryo-electron microscopy explains how a LFY-UFO complex forms on these novel DNA sites due to direct interaction of UFO with LFY and DNA. Finally, we show that this complex has a deep evolutionary origin, largely predating flowering plants. This work reveals a novel mechanism of an F-box protein in directly modulating the DNA-binding specificity of a master TF.

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Metadata

Authors
Rieu, P., Turchi, L., Thévenon, E., Zarkadas, E., Nanao, M., Chahtane, H., Tichtinsky, G., Lucas, J., Blanc-Mathieu, R., Zubieta, C., Schoehn, G., & Parcy, F.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • European Synchrotron Radiation Facility (FR)
Corresponding author
Parcy, François
ORCID
Venue
Nature Plants
Publisher
openRxiv
Published
2022-06-15
Volume
9
Issue
2
Pages
315-329
ISSN
2055-026X (ISSN-L)
DOI
10.1101/2022.06.14.495942
OpenAlex
W4318981994
Cited by
65
References
1
Citations by year
2026: 18 · 2025: 25 · 2024: 17 · 2023: 5
FWCI
11.05
Type
journal article
Language
en
Open access
Locations
  • Nature Plants (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Funders
  • Infrastructures en Biologie Santé et Agronomie (ANR-17-EURE-0003)
  • Agence Nationale de la Recherche (UAR 3518 CNRS-CEA-UGA-EMBL, ANR-17-EURE-0003, ANR-17-EURE-, ANR-17-CE20-0014, 0014-01, ANR-17, ANR-10, ANR-10-INBS-0005, ANR-10-LABX-0049)
  • Centre National de la Recherche Scientifique (UAR 3518 CNRS-CEA-UGA-EMBL)
  • French Infrastructure for Integrated Structural Biology (ANR-17-EURE-0003, ANR-10-INBS-0005, ANR-10-INBS-000502)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
Keywords
UFOs, LEAFY, F-box protein, flower development, transcriptional cofactors, cis-regulatory elements, DNA binding specificity, transcription factor-DNA complex, cryo-electron microscopy, angiosperm evolution, floral organ identity
MeSH terms
Transcription Factors, Arabidopsis, Gene Expression Regulation, Plant, Cryoelectron Microscopy, Arabidopsis Proteins, Flowers, F-Box Proteins

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