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PreprintCited by 27Epigenetics and DNA MethylationGenomics and Chromatin DynamicsChromosomal and Genetic Variations

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Uncoupled evolution of the Polycomb system and deep origin of non-canonical PRC1

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Potter, Bastiaan de, Raas, Maximilian W. D., Seidl, Michael F., Verrijzer, C. Peter, Snel, Berend·2023·Communications Biology
DOI 10.1101/2023.04.04.535607PMC PMC10638273

Preprint — published as 10.1038/s42003-023-05501-x · Paper page

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Abstract

Polycomb group (PcG) proteins modulate chromatin states to silence gene transcription in plants and animals. Most PcG proteins function as part of distinct multi-subunit Polycomb repressive complexes (PRCs). Gene repression by the Polycomb system involves chromatin compaction by canonical PRC1 (cPRC1), mono-ubiquitylation of histone H2A (H2Aub1) by non-canonical PRC1 (ncPRC1) and tri-methylation of histone H3K27 (H3K27me3) by PRC2. Prevalent models for Polycomb repression emphasize a tight functional coupling between PRC1 and PRC2. However, whether this paradigm indeed reflects the evolution and functioning of the Polycomb system remains unclear. Here, we examined the relationship between cPRC1, ncPRC1 and PRC2 through a comprehensive analysis of their presence and evolution across the entire eukaryotic tree of life. We show that both PRC1 and PRC2 were present in the Last Eukaryotic Common Ancestor (LECA), but that their subsequent evolution is uncoupled. The identification of orthologs for ncPRC1-defining subunits in unicellular relatives of animals and of fungi suggests that the origin of ncPRC1 predates that of cPRC1, and we develop a scenario for the evolution of cPRC1 from ncPRC1. Our results demonstrate the independent evolution and function of PRC1 and PRC2 and show that crosstalk between these complexes is a secondary development in evolution.

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Metadata

Authors
Potter, B. D., Raas, M. W. D., Seidl, M. F., Verrijzer, C. P., & Snel, B.
Affiliations
  • Royal Netherlands Academy of Arts and Sciences (NL)
  • Utrecht University (NL)
  • Erasmus MC (NL)
Corresponding author
Snel, Berend
ORCID
Venue
Communications Biology
Publisher
openRxiv
Published
2023-04-04
Volume
6
Issue
1
Pages
1144-1144
ISSN
2399-3642 (ISSN-L)
DOI
10.1101/2023.04.04.535607
PMC
PMC10638273
OpenAlex
W4388566557
Cited by
27
References
1
Citations by year
2026: 8 · 2025: 10 · 2024: 6 · 2023: 3
FWCI
2.39
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Communications Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (016.160.638, NWO‐Vici 016.160.638)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Keywords
Polycomb group proteins, PRC1, polycomb repressive complex 2, polycomb repressive complex, H3K27me3, gene repression, chromatin condensation, gene regulatory evolution, last eukaryotic common ancestor, eukaryotic phylogeny, unicellular eukaryotes
MeSH terms
Animals, Chromatin, Histones, Ubiquitination, Polycomb-Group Proteins, Polycomb Repressive Complex 1

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