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Cited by 8Epigenetics and DNA MethylationGenomics and Chromatin DynamicsCancer-related gene regulation

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Caenorhabditis elegans MES-3 is a highly divergent ortholog of the canonical PRC2 component SUZ12

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Snel, Berend, van den Heuvel, Sander, Seidl, Michael F.·2022·iScience
DOI 10.1016/j.isci.2022.104633PMC PMC9253717

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Abstract

Polycomb Repressive Complex 2 (PRC2) catalyzes the mono-, di-, and trimethylation of histone protein H3 on lysine 27 (H3K27), which is strongly associated with transcriptionally silent chromatin. The functional core of PRC2 is highly conserved in animals and consists of four subunits. One of these, SUZ12, has not been identified in the genetic model Caenorhabditis elegans , whereas C. elegans PRC2 contains the clade-specific MES-3 protein. Through unbiased sensitive sequence similarity searches complemented by high-quality structure predictions of monomers and multimers, we here demonstrate that MES-3 is a highly divergent ortholog of SUZ12. MES-3 shares protein folds and conserved residues of key domains with SUZ12 and is predicted to interact with core PRC2 members similar to SUZ12 in human PRC2. Thus, in agreement with previous genetic and biochemical studies, we provide evidence that C. elegans contains a diverged yet evolutionary conserved core PRC2, like other animals.

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Metadata

Authors
Snel, B., van den Heuvel, S., & Seidl, M. F.
Affiliations
Utrecht University (NL)
Corresponding author
Seidl, Michael F.
ORCID
Venue
iScience
Publisher
Elsevier BV
Published
2022-07
Volume
25
Issue
7
Pages
104633
ISSN
2589-0042 (ISSN-L)
DOI
10.1016/j.isci.2022.104633
PMC
PMC9253717
OpenAlex
W4283078593
Cited by
8
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 3 · 2023: 3
FWCI
0.57
Type
journal article
Language
en
Open access
Licence
Locations
  • iScience (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
  • NWO Earth and Life Sciences (016.160.638, 2019.017)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
Keywords
SUZ12, polycomb repressive complex 2, H3K27 methylation, H3K27, chromatin silencing, protein structure prediction, protein domain conservation, Caenorhabditis elegans

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