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PreprintCited by 6Cellular transport and secretionPlant Stress Responses and TolerancePlant Molecular Biology Research

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Regulation of adaptive growth decisions via phosphorylation of the TRAPPII complex in Arabidopsis

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Wiese, Christian, Abele, Miriam, Al, Benjamin, Altmann, Melina, Steiner, Alexander, Kalbfuß, Nils, Strohmayr, Alexander, Ravikumar, Raksha, Park, Chan Ho, Brunschweiger, Barbara, Meng, Chen, Facher, Eva, Ehrhardt, David W., Falter-Braun, Pascal, Wang, Zhi-Yong, Ludwig, Christina, Assaad, Farhah F.·2023·The Journal of Cell Biology
DOI 10.1101/2023.04.24.537966PMC PMC10983811

Preprint — published as 10.1083/jcb.202311125

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Abstract

Plants often adapt to adverse or stress conditions via differential growth. The trans-Golgi Network (TGN) has been implicated in stress responses, but it is not clear in what capacity it mediates adaptive growth decisions. In this study, we assess the role of the TGN in stress responses by exploring the interactome of the Transport Protein Particle II (TRAPPII) complex, required for TGN structure and function. We identified physical and genetic interactions between TRAPPII and shaggy-like kinases (GSK3/AtSKs). Kinase assays and pharmacological inhibition provided in vitro and in vivo evidence that AtSKs target the TRAPPII-specific subunit AtTRS120/TRAPPC9. GSK3/AtSK phosphorylation sites in AtTRS120/TRAPPC9 were mutated, and the resulting AtTRS120 phosphovariants subjected to a variety of single and multiple stress conditions in planta . The non-phosphorylatable TRS120 mutant exhibited enhanced adaptation to multiple stress conditions and to osmotic stress whereas the phosphomimetic version was less resilient. Higher order inducible trappii atsk mutants had a synthetically enhanced defect in root gravitropism. Our results suggest that the TRAPPII phosphostatus mediates adaptive responses to abiotic cues. AtSKs are multifunctional kinases that integrate a broad range of signals. Similarly, the TRAPPII interactome is vast and considerably enriched in signaling components. An AtSK-TRAPPII interaction would integrate all levels of cellular organization and instruct the TGN, a central and highly discriminate cellular hub, as to how to mobilize and allocate resources to optimize growth and survival under limiting or adverse conditions.

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Metadata

Authors
Wiese, C., Abele, M., Al, B., Altmann, M., Steiner, A., Kalbfuß, N., Strohmayr, A., Ravikumar, R., Park, C. H., Brunschweiger, B., Meng, C., Facher, E., Ehrhardt, D. W., Falter-Braun, P., Wang, Z.-Y., Ludwig, C., & Assaad, F. F.
Affiliations
  • Technical University of Munich (DE)
  • Helmholtz Munich (DE)
  • Carnegie Institution for Science (US)
  • Ludwig-Maximilians-Universität München (DE)
Corresponding author
Wiese, Christian
ORCID
Venue
The Journal of Cell Biology
Publisher
openRxiv
Published
2023-04-26
Volume
223
Issue
5
ISSN
0021-9525 (ISSN-L)
DOI
10.1101/2023.04.24.537966
PMC
PMC10983811
OpenAlex
W4393386649
Cited by
6
References
1
Citations by year
2026: 2 · 2025: 3 · 2024: 1
FWCI
1.34
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion)
  • mediaTUM (Technical University of Munich) (submittedVersion)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (INST 95/1184-1 FUGG, AS110/5-2, AS110/10-1, AS110/8-1)
  • Bayerisch-Kalifornischen Hochschulzentrum (BaCaTEC Nr. 14 [2018-2])
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM066258)
  • H2020 European Research Council (648420)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
trans-Golgi network, SHAGGY-like kinases, glycogen synthase kinase-3, protein phosphorylation, adaptive growth, abiotic stress response, Arabidopsis thaliana, protein-protein interaction network, Golgi trafficking, resource allocation

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