Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
Open accessAbstract
Long-standing models propose that plant growth responses to light or gravity are mediated by asymmetric distribution of the phytohormone auxin. Physiological studies implicated a specific transport system that relocates auxin laterally, thereby effecting differential growth; however, neither the molecular components of this system nor the cellular mechanism of auxin redistribution on light or gravity perception have been identified. Here, we show that auxin accumulates asymmetrically during differential growth in an efflux-dependent manner. Mutations in the Arabidopsis gene PIN3, a regulator of auxin efflux, alter differential growth. PIN3 is expressed in gravity-sensing tissues, with PIN3 protein accumulating predominantly at the lateral cell surface. PIN3 localizes to the plasma membrane and to vesicles that cycle in an actin-dependent manner. In the root columella, PIN3 is positioned symmetrically at the plasma membrane but rapidly relocalizes laterally on gravity stimulation. Our data indicate that PIN3 is a component of the lateral auxin transport system regulating tropic growth. In addition, actin-dependent relocalization of PIN3 in response to gravity provides a mechanism for redirecting auxin flux to trigger asymmetric growth.
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Phytochromes can regulate other auxin transporters such as PIN1, PIN3, PIN7 and PID.
“Phytochromes can also regulate other auxin transporters such as PIN1, PIN3, PIN7 and PID”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Friml et al., 2002
PIN3 gene mutation weakens auxin accumulation on the dorsal light side of the hypocotyl in Arabidopsis and leads to reduced phototropism.
“Using the auxin reporter gene DR5rev:GFP to observe the effect of PINs on the auxin distribution, PIN3 gene mutation was found to weaken auxin accumulation on the dorsal light side of the hypocotyl in Arabidopsis and lead to reduced phototropism (Friml et al., 2002).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Friml et al., 2002
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The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
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- Authors
- Friml, J., Wiśniewska, J., Benková, E., Mendgen, K., & Palme, K.
- Affiliations
- Max Planck Society (DE)
- University of Konstanz (DE)
- Corresponding author
- Palme, Klaus
- Venue
- Nature
- Published
- 2002-02
- Volume
- 415
- Issue
- 6873
- Pages
- 806-809
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2091407052
- Cited by
- 1,426
- References
- 24
- Citations by year
- 2026: 31 · 2025: 34 · 2024: 45 · 2023: 48 · 2022: 52 · 2021: 69 · 2020: 70 · 2019: 60 · 2018: 44 · 2017: 67 · 2016: 53 · 2015: 64 · 2014: 65 · 2013: 76 · 2012: 76
- FWCI
- 87.58
- Type
- journal article
- Language
- en
- Open access
- green
- nbn-resolving.de
- Licence
- cc-by-nc-nd
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- KOPS (University of Konstanz) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- PIN3, auxin efflux, gravitropism, phototropism, differential growth, auxin redistribution, plasma membrane localization, gravity sensing, Arabidopsis thaliana
- MeSH terms
- Exons, Gravitation, Indoleacetic Acids, Introns, Molecular Sequence Data, Mutation, Phenotype, Genes, Plant, Arabidopsis, Gene Expression Regulation, Plant, Hypocotyl, Physical Chromosome Mapping, Protein Transport, Arabidopsis Proteins
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