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Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins
Open accessPreprint — published as 10.1091/mbc.e23-06-0233 · Paper page
Abstract
Myosin-1s are monomeric actin-based motors that function at membranes. Myo1 is the single myosin-1 isoform in Schizosaccharomyces pombe that works redundantly with Wsp1-Vrp1 to activate the Arp2/3 complex for endocytosis. Here, we identified Ank1 as an uncharacterized cytoplasmic Myo1 binding partner. We found that in ank1Δ cells, Myo1 dramatically redistributed from endocytic patches to decorate the entire plasma membrane and endocytosis was defective. Biochemical analysis and structural predictions suggested that the Ank1 ankyrin repeats bind the Myo1 lever arm and the Ank1 acidic tail binds the Myo1 TH1 domain to prevent TH1-dependent Myo1 membrane binding. Indeed, Ank1 over-expression precluded Myo1 membrane localization and recombinant Ank1 blocked purified Myo1 liposome binding in vitro. Based on biochemical and cell biology analyses, we propose budding yeast Ank1 and human OSTF1 are functional Ank1 orthologs and that cytoplasmic sequestration by small ankyrin repeat proteins is a conserved mechanism regulating myosin-1s in endocytosis. Summary: Fission yeast long-tailed myosin-1 binds Ank1. Ank1 ankyrin repeats associate with the Myo1 lever arm and Ank1 acidic tail binds the Myo1 TH1 domain to inhibit Myo1 membrane binding. Ank1 orthologs exists in budding yeast (Ank1) and humans (OSTF1).
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Metadata
- Authors
- Willet, A. H., Chen, J.-S., Ren, L., & Gould, K. L.
- Affiliations
- Vanderbilt University (US)
- Corresponding author
- Gould, Kathleen L.
- Venue
- Molecular Biology of the Cell
- Publisher
- openRxiv
- Published
- 2023-04-27
- Volume
- 34
- Issue
- 11
- Pages
- br17-br17
- ISSN
- 1059-1524 (ISSN-L)
- PMC
- PMC10559312
- OpenAlex
- W4385497939
- Cited by
- 2
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1
- FWCI
- 0.29
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nd
- Locations
- Molecular Biology of the Cell (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion, cc-by-nd)
- Funders
- National Institute of General Medical Sciences (R35GM131799)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- ankyrin repeat proteins, endocytosis, membrane binding, ANK1, lever arm, cytoplasmic sequestration, plasma membrane localization, Saccharomyces cerevisiae, liposome binding
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