The DOI matches its Crossref record; 73 of the 73 references it cites were found in a registry or an index.
Membrane contact sites regulate vacuolar fission via sphingolipid metabolism
Open accessPreprint — published as 10.7554/elife.89938 · Paper page
Abstract
Membrane contact sites (MCSs) are junctures that perform important roles including coordinating lipid metabolism. Previous studies have indicated that vacuolar fission/fusion processes are coupled with modifications in the membrane lipid composition. However, it has been still unclear whether MCS-mediated lipid metabolism controls the vacuolar morphology. Here we report that deletion of tricalbins (Tcb1, Tcb2, Tcb3), tethering proteins at endoplasmic reticulum (ER)-plasma membrane (PM) and ER-Golgi contact sites, alters fusion/fission dynamics and causes vacuolar fragmentation in the yeast Saccharomyces cerevisiae . In addition, we show that the sphingolipid precursor phytosphingosine accumulates in tricalbin-deleted cells, triggering the vacuolar division. Detachment of the nucleus vacuole junction (NVJ), an important contact site between the vacuole and the perinuclear ER, restored vacuolar morphology in both cells subjected to high exogenous phytosphingosine and Tcb3-deleted cells, supporting that phytosphingosine transport across the NVJ induces vacuole division. Thus, our results suggest that vacuolar morphology is maintained by MCSs through the metabolism of sphingolipids.
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Here, there, and everywhere: The importance of ER membrane contact sites
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Research works printed but not linked: 1
- 10.1083/jcb.201610032 · DOI
Metadata
- Authors
- Hanaoka, K., Nishikawa, K., Ikeda, A., Schlarmann, P., Yamashita, S., Nakaji, A., Fujii, S., & Funato, K.
- Affiliations
- Hiroshima University (JP)
- Corresponding author
- Funato, Kouichi
- Venue
- bioRxiv (Cold Spring Harbor Laboratory)
- Publisher
- openRxiv
- Published
- 2023-06-22
- OpenAlex
- W4381733750
- Cited by
- 0
- References
- 73
- Type
- posted content
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by
- Locations
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- Funders
- Japan Society for the Promotion of Science (21K19088, 21K20572, 19H02922, 22K14863)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- membrane contact sites, vacuole fusion, sphingolipid metabolism, phytosphingosine, vacuole morphology, ER-plasma membrane junctions, lipid metabolism, Saccharomyces cerevisiae
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- Hanaoka, K., Nishikawa, K., Ikeda, A., Schlarmann, P., Yamashita, S., Nakaji, A., Fujii, S., & Funato, K. (2023). Membrane contact sites regulate vacuolar fission via sphingolipid metabolism. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.21.546015
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