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PreprintCited by 0Cellular transport and secretionSphingolipid Metabolism and SignalingLipid metabolism and biosynthesis

The DOI matches its Crossref record; 73 of the 73 references it cites were found in a registry or an index.

Membrane contact sites regulate vacuolar fission via sphingolipid metabolism

Open access
Hanaoka, Kazuki, Nishikawa, Kensuke, Ikeda, Atsuko, Schlarmann, Philipp, Yamashita, Sayumi, Nakaji, Aya, Fujii, Sotaro, Funato, Kouichi·2023·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2023.06.21.546015

Preprint — published as 10.7554/elife.89938 · Paper page

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Abstract

Membrane contact sites (MCSs) are junctures that perform important roles including coordinating lipid metabolism. Previous studies have indicated that vacuolar fission/fusion processes are coupled with modifications in the membrane lipid composition. However, it has been still unclear whether MCS-mediated lipid metabolism controls the vacuolar morphology. Here we report that deletion of tricalbins (Tcb1, Tcb2, Tcb3), tethering proteins at endoplasmic reticulum (ER)-plasma membrane (PM) and ER-Golgi contact sites, alters fusion/fission dynamics and causes vacuolar fragmentation in the yeast Saccharomyces cerevisiae . In addition, we show that the sphingolipid precursor phytosphingosine accumulates in tricalbin-deleted cells, triggering the vacuolar division. Detachment of the nucleus vacuole junction (NVJ), an important contact site between the vacuole and the perinuclear ER, restored vacuolar morphology in both cells subjected to high exogenous phytosphingosine and Tcb3-deleted cells, supporting that phytosphingosine transport across the NVJ induces vacuole division. Thus, our results suggest that vacuolar morphology is maintained by MCSs through the metabolism of sphingolipids.

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Crossref · 10.1101/2023.06.21.546015

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  1. 10.1083/jcb.201610032 · DOI

Metadata

Authors
Hanaoka, K., Nishikawa, K., Ikeda, A., Schlarmann, P., Yamashita, S., Nakaji, A., Fujii, S., & Funato, K.
Affiliations
Hiroshima University (JP)
Corresponding author
Funato, Kouichi
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2023-06-22
DOI
10.1101/2023.06.21.546015
OpenAlex
W4381733750
Cited by
0
References
73
Type
posted content
Language
en
Open access
Licence
cc-by
Locations
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
Funders
Japan Society for the Promotion of Science (21K19088, 21K20572, 19H02922, 22K14863)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
membrane contact sites, vacuole fusion, sphingolipid metabolism, phytosphingosine, vacuole morphology, ER-plasma membrane junctions, lipid metabolism, Saccharomyces cerevisiae

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