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PreprintCited by 17Photosynthetic Processes and MechanismsPhotoreceptor and optogenetics researchProtein Structure and Dynamics

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Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families

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Sluchanko, Nikolai N., Maksimov, Eugene G., Slonimskiy, Yury B., Varfolomeeva, Larisa A., Bukhanko, Antonina Y., Egorkin, Nikita A., Tsoraev, Georgy V., Khrenova, Maria G., Ge, Baosheng, Qin, Song, Boyko, Konstantin M., Popov, Vladimir O.·2023·International Journal of Biological Macromolecules
DOI 10.1101/2023.08.20.554024

Preprint — published as 10.1016/j.ijbiomac.2023.127874 · Paper page

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Abstract

The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non-photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP and rationalize the observed differences in OCP/FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering.

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Metadata

Authors
Sluchanko, N. N., Maksimov, E. G., Slonimskiy, Y. B., Varfolomeeva, L. A., Bukhanko, A. Y., Egorkin, N. A., Tsoraev, G. V., Khrenova, M. G., Ge, B., Qin, S., Boyko, K. M., & Popov, V. O.
Affiliations
  • Russian Academy of Sciences (RU)
  • Lomonosov Moscow State University (RU)
  • China University of Petroleum, East China (CN)
  • Chinese Academy of Sciences (CN)
Corresponding author
  • Sluchanko, Nikolai N.
  • Qin, Song
ORCID
Venue
International Journal of Biological Macromolecules
Publisher
openRxiv
Published
2023-08-20
Volume
254
Issue
Pt 2
Pages
127874-127874
ISSN
0141-8130 (ISSN-L)
DOI
10.1101/2023.08.20.554024
OpenAlex
W4388440528
Cited by
17
References
1
Citations by year
2026: 4 · 2025: 6 · 2024: 6 · 2023: 1
FWCI
1.51
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • Knowledge Repository of Yantai Institute of Coastal Zone Research, CAS (Yantai Institute of Coastal Zone Research) (submittedVersion)
Funders
  • Ministry of Education and Science of the Russian Federation
  • Russian Science Foundation (21-44-00005)
  • Moscow State University of Geodesy and Cartography
  • Ministry of Science and Higher Education of the Russian Federation (075–15‐2021–1354, 075-15-)
  • Lomonosov Moscow State University
  • Russian Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
orange carotenoid protein, cyanobacteria, crystal structure, phycobilisomes, non-photochemical quenching, photoprotection, ketocarotenoids, chalcogen bonding, echinenone, carotenoid biosynthesis, optogenetics, carotenoid delivery
MeSH terms
Bacterial Proteins, Carotenoids, Spectrum Analysis, Phycobilisomes, Synechocystis

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