CoChat
Sign Up
PreprintCited by 31Malaria Research and ControlMonoclonal and Polyclonal Antibodies ResearchBlood groups and transfusion

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Analysis of the Diverse Antigenic Landscape of the Malaria Invasion Protein RH5 Identifies a Potent Vaccine-Induced Human Public Antibody Clonotype

Open access
Barrett, Jordan R., Pipini, Dimitra, Wright, Nathan D., Cooper, Andrew J. R., Gorini, Giacomo, Quinkert, Doris, Lias, Amelia M., Davies, Hannah, Rigby, Cassandra, Aleshnick, Maya, Williams, Barnabas G., Bradshaw, William J., Paterson, Neil G., Martinson, Thomas, Kirtley, Payton, Picard, Luc, Wiggins, Christine D., Donnellan, Francesca R., King, Lloyd D. W., Wang, Lawrence T., Popplewell, Jonathan F., Silk, Sarah E., Swain, Jed de Ruiter, Skinner, Katherine, Kotraiah, Vinayaka, Noe, Amy R., MacGill, Randall S., King, C. Richter, Birkett, Ashley J., Soisson, Lorraine A., Minassian, Angela M., Lauffenburger, Douglas A., Miura, Kazutoyo, Long, Carole A., Wilder, Brandon K., Koekemoer, Lizbé, Tan, Joshua, Nielsen, Carolyn M., McHugh, Kirsty, Draper, Simon J.·2023·Cell
DOI 10.1101/2023.10.04.560576PMC PMC11380582

Preprint — published as 10.1016/j.cell.2024.06.015

Open this paper in CoChat — free account

Abstract

SUMMARY: The highly conserved and essential Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has emerged as the leading target for vaccines that seek to protect against the disease-causing blood-stage of malaria. However, the features of the human vaccine-induced antibody response that confer highly potent inhibition of malaria parasite invasion into red blood cells are not well defined. Here we characterize over 200 human IgG monoclonal antibodies induced by the most advanced PfRH5 vaccine. We define the antigenic landscape of this molecule, and establish epitope specificity, antibody association rate and intra-PfRH5 antibody interactions are key determinants of functional anti-parasitic potency. In addition, we identify a germline gene combination that results in an exceptionally potent class of antibody and demonstrate its prophylactic potential to protect against P. falciparum parasite challenge in vivo . This comprehensive dataset provides a framework to guide rational design of next-generation vaccines and prophylactic antibodies to protect against blood-stage malaria.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2023.10.04.560576

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 66

  1. 10.1016/j.chom.2018.06.008 · DOI
  2. 10.1038/s41590-018-0228-6 · DOI
  3. 10.1016/S0140-6736(21)00943-0 · DOI
  4. 10.1016/S0140-6736(15)60721-8 · DOI
  5. 10.1056/NEJMoa2203067 · DOI
  6. 10.1038/ncomms1615 · DOI
  7. 10.1038/nature10606 · DOI
  8. 10.1016/j.pt.2020.04.003 · DOI
  9. 10.1038/s41564-022-01261-2 · DOI
  10. 10.1371/journal.pbio.3000490 · DOI
  11. 10.1016/j.chom.2014.11.017 · DOI
  12. 10.1016/j.medj.2021.03.014 · DOI
  13. 10.1038/s41467-019-09894-4 · DOI
  14. 10.3389/fimmu.2018.00524 · DOI
  15. 10.1038/s41541-018-0071-7 · DOI
  16. 10.1172/jci.insight.96381 · DOI
  17. 10.1016/j.medj.2023.07.003 · DOI
  18. 10.1038/nature13715 · DOI
  19. 10.1038/s41586-018-0779-6 · DOI
  20. 10.4049/jimmunol.1302045 · DOI
  21. 10.7554/eLife.20383 · DOI
  22. 10.1111/cmi.13030 · DOI
  23. 10.1016/j.cell.2019.05.025 · DOI
  24. 10.1038/s41467-022-28601-4 · DOI
  25. 10.1101/2022.09.23.509221 · DOI
  26. 10.1038/s41586-019-0879-y · DOI · Paper page
  27. 10.1016/j.cell.2020.11.043 · DOI
  28. 10.7554/eLife.04187 · DOI
  29. 10.1371/journal.ppat.1002991 · DOI
  30. 10.1038/s41598-020-67877-8 · DOI
  31. 10.1038/nature11174 · DOI
  32. 10.1172/jci.insight.143958 · DOI
  33. 10.1038/s41467-023-37890-2 · DOI
  34. 10.7554/eLife.21347 · DOI
  35. 10.1111/j.1365-3024.1987.tb00483.x · DOI
  36. 10.1038/s41564-019-0462-1 · DOI
  37. 10.1172/jci.insight.163859 · DOI
  38. 10.1016/S1473-3099(22)00428-5 · DOI
  39. 10.1093/nar/gkab1061 · DOI · Paper page
  40. 10.1016/j.ijpara.2016.12.001 · DOI
  41. 10.1038/srep30357 · DOI
  42. 10.1074/mcp.O113.028357 · DOI
  43. 10.1016/j.xcrm.2021.100207 · DOI
  44. 10.1016/j.jim.2007.09.017 · DOI
  45. 10.1038/nbt.1601 · DOI
  46. 10.3389/fimmu.2019.01254 · DOI
  47. 10.1128/mSphere.00647-21 · DOI
  48. 10.1128/IAI.73.6.3677-3685.2005 · DOI
  49. 10.1016/j.celrep.2019.03.020 · DOI
  50. 10.1111/j.1744-7348.1939.tb06990.x · DOI · Paper page
  51. 10.1107/S2059798320014114 · DOI
  52. 10.1107/S2059798317017235 · DOI
  53. 10.1107/S0021889809045701 · DOI
  54. 10.1093/bioinformatics/btr535 · DOI
  55. 10.1107/S1600576715004847 · DOI
  56. 10.1107/S0907444903008126 · DOI
  57. 10.1107/S0907444910045749 · DOI · Paper page
  58. 10.1107/S0021889807021206 · DOI · Paper page
  59. 10.1093/nar/gky949 · DOI · Paper page
  60. 10.1107/S0907444910007493 · DOI · Paper page
  61. 10.1107/S0907444911001314 · DOI
  62. 10.1107/S2052252514009324 · DOI
  63. 10.1107/S2059798316014534 · DOI
  64. 10.1038/s41541-017-0028-2 · DOI
  65. 10.1038/s41467-017-01924-3 · DOI
  66. 10.4269/ajtmh.19-0094 · DOI

Metadata

Authors
Barrett, J. R., Pipini, D., Wright, N. D., Cooper, A. J. R., Gorini, G., Quinkert, D., Lias, A. M., Davies, H., Rigby, C., Aleshnick, M., Williams, B. G., Bradshaw, W. J., Paterson, N. G., Martinson, T., Kirtley, P., Picard, L., Wiggins, C. D., Donnellan, F. R., King, L. D. W., Wang, L. T., Popplewell, J. F., Silk, S. E., Swain, J. D. R., Skinner, K., Kotraiah, V., Noe, A. R., MacGill, R. S., King, C. R., Birkett, A. J., Soisson, L. A., Minassian, A. M., Lauffenburger, D. A., Miura, K., Long, C. A., Wilder, B. K., Koekemoer, L., Tan, J., Nielsen, C. M., McHugh, K., & Draper, S. J.
Affiliations
  • University of Oxford (GB)
  • National Institutes of Health (US)
  • Vaccine and Gene Therapy Institute (US)
  • Diamond Light Source (GB)
  • Massachusetts Institute of Technology (US)
  • Carterra (United States) (US)
  • Leidos (United States) (US)
  • Program for Appropriate Technology in Health (US)
  • United States Agency for International Development (US)
  • National Institute of Allergy and Infectious Diseases (US)
Corresponding author
Draper, Simon J.
ORCID
Venue
Cell
Publisher
openRxiv
Published
2023-10-04
Volume
187
Issue
18
Pages
4964-4980.e21
ISSN
0092-8674 (ISSN-L)
DOI
10.1101/2023.10.04.560576
PMC
PMC11380582
OpenAlex
W4400978373
Cited by
31
References
1
Citations by year
2026: 8 · 2025: 11 · 2024: 12
FWCI
12.84
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion)
Funders
  • United States Agency for International Development (AID-OAA-C-15-00071, 7200AA20C00017)
  • Bill and Melinda Gates Foundation (INV-005170, INV-027520)
  • Division of Intramural Research, National Institute of Allergy and Infectious Diseases
  • NHS Blood and Transplant
  • Wellcome Trust (106917/Z/15/Z, 209200/Z/17/Z, Z/17/Z, /Z/15/Z)
  • Diamond Light Source
  • Leidos
  • National Institute for Health and Care Research (209200/Z/17/Z)
  • Department of Health and Social Care
  • University of Oxford
  • European Commission (733273, HORIZON2020)
  • National Institutes of Health (106917/Z/15/Z, 209200/Z/17/Z, Z99 AI999999)
  • Horizon 2020 Framework Programme (733273)
  • Medical Research Council (MR/N013468/1, MR/X012085/1, MR/N013468/)
  • Horizon 2020
  • National Institute of Allergy and Infectious Diseases
  • Office of Extramural Research, National Institutes of Health
  • NHLBI Division of Intramural Research
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
  • Health Sciences › Medicine › Hematology › Blood groups and transfusion
Keywords
PfRH5, Plasmodium falciparum, malaria vaccine, human monoclonal antibodies, epitope specificity, blood-stage malaria
MeSH terms
Animals, Antibodies, Monoclonal, Antibodies, Protozoan, Epitopes, Antigens, Protozoan, Carrier Proteins, Erythrocytes, Female, Humans, Immunoglobulin G, Plasmodium falciparum, Protozoan Proteins, Malaria, Falciparum, Malaria Vaccines, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review