CoChat
Sign Up
PreprintCited by 16Monoclonal and Polyclonal Antibodies ResearchT-cell and B-cell ImmunologyProtein Structure and Dynamics

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

CD5L associates with IgM via the J chain

Open access
Wang, Yuxin, Su, Chen, Ji, Chengong, Xiao, Junyu·2023·Nature Communications
DOI 10.1101/2023.12.18.572142PMC PMC11437284

Preprint — published as 10.1038/s41467-024-52175-y · Paper page

Open this paper in CoChat — free accountOpen PDF

Abstract

CD5 antigen-like (CD5L), also known as Spα or AIM (Apoptosis inhibitor of macrophage), emerges as an integral component of serum immunoglobulin M (IgM). However, the molecular mechanism underlying the interaction between IgM and CD5L has remained elusive. In this study, we present a cryo-electron microscopy structure of the IgM pentamer core in complex with CD5L. Our findings reveal that CD5L binds to the joining chain (J chain) in a Ca 2+ -dependent manner and further links to IgM via a disulfide bond. We further show that CD5L reduces IgM binding to the mucosal transport receptor pIgR, but does not impact the binding of the IgM-specific receptor FcμR. Additionally, CD5L does not affect IgM-mediated complement activation. These results offer new insights into our understanding of IgM and shed light on the functions of the IgM–CD5L complex in the immune system.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2023.12.18.572142

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 45

  1. 10.1084/jem.65.3.393 · DOI
  2. 10.1002/elps.1150140137 · DOI
  3. 10.1016/0022-1759(94)90284-4 · DOI
  4. 10.1074/jbc.272.10.6151 · DOI
  5. 10.1084/jem.189.2.413 · DOI
  6. 10.1046/j.1365-2567.2000.00903.x · DOI
  7. 10.1002/1522-2683(200204)23:7/8<1203::AID-ELPS1203>3.0.CO;2-1 · DOI
  8. 2023, CD5L is a canonical component of circulatory IgM, Proceedings of the National Academy of Sciences, 120, e2311265120, 10.1073/pnas.2311265120 · DOI
  9. 10.1189/jlb.3RU0215-074R · DOI
  10. 2018, AIM associated with the IgM pentamer: attackers on stand-by at aircraft carrier, Cellular & molecular immunology, 15, 563, 10.1038/cmi.2017.141 · DOI
  11. 10.1016/j.cell.2004.09.032 · DOI
  12. 10.1074/jbc.M505042200 · DOI
  13. 10.1038/cmi.2014.12 · DOI
  14. 10.1016/j.intimp.2019.03.057 · DOI
  15. Maehara, N. , et al. AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke. Cell Reports 36 (2021). · DOI
  16. 10.1038/nm.4012 · DOI
  17. 10.1016/j.celrep.2014.08.058 · DOI
  18. 10.1016/S0002-9440(10)63880-1 · DOI
  19. 10.3892/etm.2020.8497 · DOI
  20. 10.1016/j.cell.2015.10.068 · DOI
  21. 10.1016/j.cmet.2010.04.013 · DOI
  22. 10.1016/j.cmet.2005.02.002 · DOI
  23. 10.1681/ASN.2019100987 · DOI
  24. 10.1016/0968-0004(94)90165-1 · DOI
  25. 10.1615/CritRevImmunol.v24.i1.10 · DOI
  26. 10.26508/lsa.201900502 · DOI
  27. 10.1038/srep35251 · DOI
  28. 10.1126/sciadv.aau1199 · DOI
  29. 10.1073/pnas.1901841116 · DOI
  30. 10.1126/science.aaz5425 · DOI
  31. 10.1016/j.str.2021.01.002 · DOI
  32. 10.1038/s41467-022-34090-2 · DOI
  33. 10.1126/science.aaz5807 · DOI
  34. 10.1038/s41422-020-0336-3 · DOI
  35. 10.7554/eLife.56098 · DOI
  36. 10.1084/jem.20091107 · DOI
  37. 10.1038/s41586-023-05835-w · DOI
  38. 10.1038/80886 · DOI
  39. 1994, Mapping of amino acid residues in the C mu 3 domain of mouse IgM important in macromolecular assembly and complement-dependent cytolysis, J Immunol, 152, 1206, 10.4049/jimmunol.152.3.1206 · DOI
  40. 10.1038/s41467-023-38320-z · DOI
  41. 10.1146/annurev.iy.03.040185.002233 · DOI
  42. 2017, cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination, Nature methods, 14, 290, 10.1038/nmeth.4169 · DOI · Paper page
  43. 10.1002/PRO.3943 · DOI
  44. 10.1107/S0907444910007493 · DOI · Paper page
  45. 10.1107/S0907444909052925 · DOI · Paper page

Metadata

Authors
Wang, Y., Su, C., Ji, C., & Xiao, J.
Affiliations
Peking University (CN)
Corresponding author
Xiao, Junyu
ORCID
Venue
Nature Communications
Publisher
openRxiv
Published
2023-12-18
Volume
15
Issue
1
Pages
8397-8397
ISSN
2041-1723 (ISSN-L)
DOI
10.1101/2023.12.18.572142
PMC
PMC11437284
OpenAlex
W4402903351
Cited by
16
References
1
Citations by year
2026: 11 · 2025: 4 · 2024: 1
FWCI
4.06
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Nature Communications (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Greater South Information System
Funders
  • National Natural Science Foundation of China (32325018)
  • Peking University
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
CD5L, AIM, IgM pentamer, J chain, polymeric immunoglobulin receptor, FcμR, complement activation, calcium-dependent binding, disulfide bonds, serum immunoglobulin M, protein-protein interaction, cryo-EM structure
MeSH terms
Calcium, Humans, Immunoglobulin M, Immunoglobulin J-Chains, Protein Binding, Cryoelectron Microscopy

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review