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PreprintCited by 25Lipid metabolism and biosynthesisPlant Stress Responses and TolerancePlant-Microbe Interactions and Immunity

Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress

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Scholz, Patricia, Doner, Nathan M., Gutbrod, Katharina, Herrfurth, Cornelia, Niemeyer, Philipp, Lim, Magdiel S. S., Blersch, Katharina, Schmitt, Kerstin, Valerius, Oliver, Shanklin, John, Feussner, Ivo, Dörmann, Peter, Braus, Gerhard H., Mullen, Robert T., Ischebeck, Till·2024·PLANT PHYSIOLOGY
DOI 10.1101/2024.02.09.579702PMC PMC11823117

Preprint — published as 10.1093/plphys/kiae274

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Abstract

Plants must cope with a variety of stressors during their life cycle, and the adaptive responses to these environmental cues involve all cellular organelles. Among them, comparatively little is known about the contribution of cytosolic lipid droplets (LDs) and their core set of neutral lipids and associated surface proteins to the rewiring of cellular processes in response to stress. Here, we analyzed the changes that occur in the lipidome and proteome of Arabidopsis leaves after pathogen infection with Botrytis cinerea or Pseudomonas syringae , or after heat stress. Analyses were carried out in wild-type plants and the oil-rich double mutant tgd1-1 sdp1-4 that allowed for an allied study of the LD proteome in stressed leaves. Using liquid chromatography-tandem mass spectrometry-based methods, we show that a hyperaccumulation of the primary LD core lipid triacylglycerol is a general response to stress and that acyl chain and sterol composition are remodeled during cellular adaptation. Likewise, comparative analysis of the LD protein composition in stress-treated leaves highlighted the plasticity of the LD proteome as part of the general stress response. We further identified at least two additional LD-associated proteins, whose localization to LDs in leaves was confirmed by confocal microscopy of fluorescent protein fusions. Taken together, these results highlight LDs as dynamic contributors to the cellular adaptation processes that underlie how plants respond to environmental stress. One sentence summary: Biotic and heat stress strongly alters the lipidome and proteome of Arabidopsis leaves including the proteome of lipid droplets.

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Authors
Scholz, P., Doner, N. M., Gutbrod, K., Herrfurth, C., Niemeyer, P., Lim, M. S. S., Blersch, K., Schmitt, K., Valerius, O., Shanklin, J., Feussner, I., Dörmann, P., Braus, G. H., Mullen, R. T., & Ischebeck, T.
Affiliations
  • University of Göttingen (DE)
  • University of Guelph (CA)
  • University of Bonn (DE)
  • University of Münster (DE)
  • Brookhaven National Laboratory (US)
Corresponding author
  • Scholz, Patricia
  • Doner, Nathan M.
  • Ischebeck, Till
ORCID
Venue
PLANT PHYSIOLOGY
Publisher
openRxiv
Published
2024-02-14
Volume
197
Issue
2
ISSN
0032-0889 (ISSN-L)
DOI
10.1101/2024.02.09.579702
PMC
PMC11823117
OpenAlex
W4398250133
Cited by
25
References
1
Citations by year
2026: 11 · 2025: 13 · 2024: 1
FWCI
4.00
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • PLANT PHYSIOLOGY (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
Funders
  • U.S. Department of Energy (DESC0016536)
  • Ontario Council on Graduate Studies, Council of Ontario Universities
  • Deutsche Forschungsgemeinschaft (INST 186/1167‐1, IS 273/10‐1, IRTG2172, IRTG 2172 “PRoTECT", INST: 186/1230-1 FUGG)
  • Studienstiftung des Deutschen Volkes
  • Office of Science (DESC0016536)
  • Natural Sciences and Engineering Research Council of Canada (rgpin-2018-04629, RGPIN 2018)
  • Brookhaven National Laboratory
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
Keywords
lipid droplets, Arabidopsis thaliana, leaf proteome, Botrytis cinerea, Pseudomonas syringae, pathogen infection, heat stress, lipid remodeling, sterols, acyl chain composition, lipid droplet proteins, plant stress response
MeSH terms
Lipidomics, Plant Diseases, Arabidopsis, Plant Leaves, Heat-Shock Response, Botrytis, Proteome, Arabidopsis Proteins, Pseudomonas syringae, Lipid Droplets

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