The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structural Requirements for Activity of Mind bomb1 in Notch Signaling
Open accessPreprint — published as 10.1016/j.str.2024.07.011 · Paper page
Abstract
Mind bomb 1 (MIB1) is a RING E3 ligase that ubiquitinates Notch ligands, a necessary step for induction of Notch signaling. The structural basis for binding of the JAG1 ligand by the N-terminal region of MIB1 is known, yet how the ankyrin (ANK) and RING domains of MIB1 cooperate to catalyze ubiquitin transfer from E2∼Ub to Notch ligands remains unclear. Here, we show that the third RING domain and adjacent coiled coil region of MIB1 (ccRING3) drives MIB1 dimerization and that ubiquitin transfer activity of MIB1 relies solely on RING3. We report x-ray crystal structures of a UbcH5B-ccRING3 complex as a fusion protein and of the ANK region. Directly tethering the N-terminal region to ccRING3 forms a minimal MIB1 protein, which is sufficient to induce a Notch response in receiver cells. Together, these studies define the functional elements of an E3 ligase needed for ligands to induce a Notch signaling response.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 43
- 10.1126/science.284.5415.770 · DOI · Paper page
- 10.1006/scdb.1998.0266 · DOI
- 10.1152/physrev.00005.2017 · DOI
- 10.1038/30756 · DOI
- 10.1073/pnas.98.1.229 · DOI
- 10.1128/MCB.21.22.7761-7774.2001 · DOI
- 10.1016/j.cell.2005.12.037 · DOI
- 10.1016/j.cell.2006.01.035 · DOI · Paper page
- 10.1038/nsmb.1938 · DOI
- 10.1016/j.semcdb.2012.01.011 · DOI
- 10.1242/dev.125.23.4637 · DOI
- 10.1016/S1534-5807(02)00409-4 · DOI
- 10.1016/S1534-5807(01)00092-2 · DOI
- 10.1083/jcb.201105166 · DOI
- 10.1371/journal.pbio.0030096 · DOI
- 10.1074/jbc.M501631200 · DOI
- 10.1016/j.molcel.2015.01.019 · DOI
- 10.1371/journal.pone.0001221 · DOI
- 10.1016/j.mod.2005.06.005 · DOI
- 10.1242/dev.01922 · DOI
- J.-Y., 2012, Mind Bomb-1 in Dendritic Cells Is Specifically Required for Notch-mediated T Helper Type2 Differentiation, PLoS ONE, 7, e36359, 10.1371/journal.pone.0036359 · DOI
- 10.1084/jem.20081344 · DOI
- 10.1128/MCB.00436-08 · DOI
- 10.1038/nm.3046 · DOI
- 10.1083/jcb.201107021 · DOI
- 10.7554/eLife.12950 · DOI
- 10.1038/emboj.2013.223 · DOI
- 2019, The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex, Cell Rep, 29, 3785, 10.1016/j.celrep.2019.11.064 · DOI
- 10.1038/cr.2011.199 · DOI
- 10.1152/ajpcell.00214.2009 · DOI
- 10.1038/nsmb.2379 · DOI · Paper page
- 10.1074/jbc.M804753200 · DOI
- 10.1038/s41467-020-15783-y · DOI
- 2022, ColabFold: making protein folding accessible to all, Nature Methods, 19, 679, 10.1038/s41592-022-01488-1 · DOI · Paper page
- 10.1128/MCB.20.20.7505-7515.2000 · DOI
- 10.1242/dev.01825 · DOI
- 10.1016/j.ydbio.2003.11.010 · DOI
- 10.1107/S0907444906045975 · DOI
- 10.1107/S0907444904019158 · DOI · Paper page
- 10.1107/S0907444909052925 · DOI · Paper page
- 10.1016/S0076-6879(97)76066-X · DOI · Paper page
- 10.1371/journal.pone.0006613 · DOI
- 10.1093/nar/gkw408 · DOI · Paper page
Metadata
- Authors
- Cao, R., Gozlan, O., Tveriakhina, L., Zhou, H., Jiang, H., Cole, P. A., Aster, J. C., Sprinzak, D., & Blacklow, S. C.
- Affiliations
- Harvard University (US)
- Tel Aviv University (IL)
- Brigham and Women's Hospital (US)
- Corresponding author
- Blacklow, Stephen C.
- ORCID
- Venue
- Structure
- Publisher
- openRxiv
- Published
- 2024-03-03
- Volume
- 32
- Issue
- 10
- Pages
- 1667-1676.e5
- ISSN
- 0969-2126 (ISSN-L)
- PMC
- PMC12094784
- OpenAlex
- W4401419802
- Cited by
- 7
- References
- 1
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Structure (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (submittedVersion, cc-by-nc-nd)
- Funders
- Charles H. Revson Foundation
- Springworks Therapeutics
- Deutsche Forschungsgemeinschaft
- United States-Israel Binational Science Foundation (2017245)
- National Institutes of Health (CA74305)
- Ludwig Center at Harvard
- National Cancer Institute (R35CA220340, R01CA272484, R01CA074305)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Notch signaling, RING E3 ubiquitin ligase, protein dimerization, UbcH5b, ankyrin domain, crystal structure, protein structure-function relationship, Drosophila
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
