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PreprintCited by 2Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsNuclear Structure and Function

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The chromokinesin Kid (KIF22) forms a homodimer, moves processively along microtubules and transports double-stranded DNA

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Niwa, Shinsuke, Furusaki, Natsuki, Kita, Tomoki, Suzuki, Yuki, Chiba, Kyoko·2024·eLife
DOI 10.1101/2024.03.13.584902

Preprint — published as 10.7554/elife.102828 · Paper page

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Abstract

During prometaphase in mitosis, chromosomes are pushed toward the spindle equator. The chromokinesin Kid, also known as KIF22, moves chromosomes along spindle microtubules during prometaphase. Kid has long been considered a monomeric and non-processive motor, different from typical kinesins. In this study, we demonstrate that the full-length Kid forms a homodimer and moves processively along microtubules. A conserved coiled-coil domain within the stalk region of Kid is sufficient for homodimer formation and is required for the processivity of Kid. Furthermore, the neck linker and coiled-coil domains of Kid could add processive activity to the motor domain of KIF1A, suggesting that Kid contains a functional neck linker and dimerization capability, a prerequisite for the processivity of kinesin motor domains. The full-length Kid, containing a helix-hairpin-helix domain, can transport double-stranded DNA along microtubules in vitro. Alphafold3 prediction suggests that the dimerization of Kid stabilizes the association with DNA. These findings collectively suggest the reclassification of Kid as a processive and dimeric motor that transports DNA along microtubules.

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Metadata

Authors
Niwa, S., Furusaki, N., Kita, T., Suzuki, Y., & Chiba, K.
Affiliations
  • Tohoku University (JP)
  • Mie University (JP)
Corresponding author
  • Niwa, Shinsuke
  • Chiba, Kyoko
ORCID
Venue
eLife
Publisher
openRxiv
Published
2024-03-13
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.1101/2024.03.13.584902
OpenAlex
W4392813782
Cited by
2
References
5
Citations by year
2024: 2
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
KIF22, chromokinesin, homodimerization, microtubule motility, coiled-coil domain, neck linker, DNA transport, double-stranded DNA, mitotic spindle

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