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Intrinsically disordered region amplifies membrane remodeling to augment selective ER-phagy
Open accessPreprint — published as 10.1073/pnas.2408071121 · Paper page
Abstract
Intrinsically disordered regions (IDRs) play a pivotal role in organellar remodeling. They transduce signals across membranes, scaffold signaling complexes, and mediate vesicular traffic. Their functions are regulated by constraining conformational ensembles through specific intra- and inter-molecular interactions, physical tethering, and post-translational modifications. The ER-phagy receptor FAM134B/RETREG1, known for its Reticulon homology domain (RHD), includes a substantial C-terminal intrinsically disorder region (IDR) housing the LC3 interacting (LIR) motif. Beyond engaging the autophagic machinery, the function of the FAM134B-IDR is unclear. Here, we investigate the characteristics of the FAM134B-IDR by extensive modeling and molecular dynamics (MD) simulations. We present detailed structural models for the IDR, mapping its conformational landscape in solution and membrane-anchored configurations. Our analysis reveals that depending on the membrane anchor, the IDRs collapse onto the membrane and induce positive membrane curvature to varying degrees. The charge patterns underlying this Janus-like behavior are conserved across other ER-phagy receptors. We found that IDRs alone are sufficient to sense curvature. When combined with RHDs, they intensify membrane remodeling and drive efficient protein clustering, leading to faster budding, thereby amplifying RHD remodeling functions. Our simulations provide a new perspective on IDRs of FAM134B, their Janus-like membrane interactions, and the resulting modulatory functions during large-scale ER remodeling.
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Highly accurate protein structure prediction with AlphaFold
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Metadata
- Authors
- Poveda-Cuevas, S. A., Lohachova, K., Dikic, I., Hummer, G., & Bhaskara, R. M.
- Affiliations
- Goethe University Frankfurt (DE)
- Corresponding author
- Bhaskara, Ramachandra M.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- openRxiv
- Published
- 2024-03-29
- Volume
- 121
- Issue
- 44
- Pages
- e2408071121-e2408071121
- ISSN
- 0027-8424 (ISSN-L)
- PMC
- PMC11536123
- OpenAlex
- W4403761302
- Cited by
- 18
- References
- 1
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 3
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) (submittedVersion, cc-by-nc-nd)
- Funders
- Deutsche Forschungsgemeinschaft (259130777)
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- intrinsically disordered regions, ER-phagy, FAM134B, membrane curvature sensing, membrane remodeling, autophagy receptors, protein clustering, membrane budding, conformational landscape
- MeSH terms
- Protein Domains, Autophagy, Cell Membrane, Endoplasmic Reticulum, Humans, Membrane Proteins, Intracellular Signaling Peptides and Proteins, Molecular Dynamics Simulation, Intrinsically Disordered Proteins
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