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Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplast
Open accessPreprint — published as 10.1038/s41467-024-54790-1
Abstract
Most angiosperm plants recognise the flg22 epitope in bacterial flagellin via homologs of cell surface receptor FLS2 and mount pattern-triggered immune responses. However, flg22 is buried within the flagellin protein indicating that proteases might be required for flg22 release. Here, we demonstrate the extracellular subtilase SBT5.2 not only releases flg22, but also inactivates the immunogenicity of flagellin and flg22 by cleaving within the flg22 epitope, consistent with previous reports that flg22 is unstable in the apoplast. The prolonged lifetime of flg22 in sbt5.2 mutant plants results in increased bacterial immunity in priming assays, indicating that SBT5.2 counterbalances flagellin immunogenicity to provide spatial-temporal control and restrict costly immune responses and that bacteria take advantage of the host proteolytic machinery to avoid detection by flagellin having a protease-sensitive flg22 epitope.
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Metadata
- Authors
- Buscaill, P., Sanguankiattichai, N., Kaschani, F., Huang, J., Mooney, B. C., Li, Y., Lyu, J., Sueldo, D., Kaiser, M., & van der Hoorn, R. A. L.
- Affiliations
- University of Bristol (GB)
- University of Oxford (GB)
- University of Duisburg-Essen (DE)
- Chinese Academy of Sciences (CN)
- Norwegian University of Science and Technology (NO)
- Corresponding author
- van der Hoorn, Renier A. L.
- ORCID
- Venue
- Nature Communications
- Publisher
- openRxiv
- Published
- 2024-05-24
- Volume
- 15
- Issue
- 1
- Pages
- 10431-10431
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC11608315
- OpenAlex
- W4404873322
- Cited by
- 24
- References
- 1
- Citations by year
- 2026: 17 · 2025: 6 · 2024: 1
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (32070289)
- Chinese Academy of Sciences
- Biotechnology and Biological Sciences Research Council (BB/015128/1, DDT00230, BB/R017913/1, 101019324, BB/R017913/3)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
- Keywords
- flagellin, FLS2, pattern-triggered immunity, proteolytic processing, immune evasion, bacterial flagellin, host proteases
- MeSH terms
- Epitopes, Flagellin, Mutation, Protein Kinases, Subtilisins, Arabidopsis, Arabidopsis Proteins, Plant Immunity
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