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PreprintCited by 6Retinal Development and DisordersRetinal Diseases and TreatmentsRetinal Imaging and Analysis

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Energetics of the outer retina II: Calculation of a spatio-temporal energy budget in retinal pigment epithelium and photoreceptor cells based on quantification of cellular processes

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Kiel, Christina, Prins, Stella, Foss, Alexander JE, Luthert, Philip J·2024·PLoS ONE
DOI 10.1101/2024.05.28.596166PMC PMC11771881

Preprint — published as 10.1371/journal.pone.0311169 · Paper page

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Abstract

The outer retina (OR) is highly energy demanding. Impaired energy metabolism combined with high demands are expected to cause energy insufficiencies that make the OR susceptible to complex blinding diseases such as age-related macular degeneration (AMD). Here, anatomical, physiological and quantitative molecular data were used to calculate the ATP expenditure of the main energy-consuming processes in three cell types of the OR for the night and two different periods during the day. The predicted energy demands in a rod dominated (perifovea) area are 1.33 × 10 13 ATP/s/mm 2 tissue in the night and 6.53 × 10 12 ATP/s/mm 2 tissue during the day with indoor light conditions. For a cone-dominated foveal area the predicted energy demands are 6.44 × 10 12 ATP/s/mm 2 tissue in the night and 6.75 × 10 12 ATP/s/mm 2 tissue with indoor light conditions during daytime. We propose the likely need of for diurnal/circadian shifts in energy demands to efficiently stagger all energy consuming processes. Our data provide insights into vulnerabilities in the aging OR and suggest that diurnal constraints may be important when considering therapeutic interventions to optimize metabolism.

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Metadata

Authors
Kiel, C., Prins, S., Foss, A. J., & Luthert, P. J.
Affiliations
  • University of Pavia (IT)
  • University College London (GB)
  • Nottingham University Hospitals NHS Trust (GB)
  • NIHR Moorfields Biomedical Research Centre (GB)
Corresponding author
Kiel, Christina
ORCID
Venue
PLoS ONE
Publisher
openRxiv
Published
2024-06-01
Volume
20
Issue
1
Pages
e0311169-e0311169
ISSN
1932-6203 (ISSN-L)
DOI
10.1101/2024.05.28.596166
PMC
PMC11771881
OpenAlex
W4406875952
Cited by
6
References
2
Citations by year
2026: 4 · 2025: 1 · 2024: 1
FWCI
1.49
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Moorfields Eye Charity (GR001345, Grant GR001345)
Topics
  • Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Retinal Imaging and Analysis
Keywords
outer retina, retinal energy metabolism, retinal pigment epithelium, photoreceptors, energy balance, circadian rhythm, diurnal variation, age-related macular degeneration, rod and cone photoreceptors, perifovea, fovea, retinal aging
MeSH terms
Adenosine Triphosphate, Circadian Rhythm, Energy Metabolism, Humans, Light, Retinal Pigment Epithelium

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