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PreprintCited by 3Bacterial Genetics and BiotechnologyMicrotubule and mitosis dynamicsBiochemical and Structural Characterization

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Tubules, rods and spirals: diverse modes of SepF-FtsZ assembling

Choudhury, Jagrity, Chaudhuri, Barnali N.·2024·Cytoskeleton
DOI 10.1101/2024.07.18.604210

Preprint — published as 10.1002/cm.21975 · Paper page

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Abstract

Z-ring formation by FtsZ, the master assembler of divisome, is a key step in bacterial cell division. Both formation and membrane anchoring of the Z-ring requires assistance of a number of Z-ring binding proteins, such as FtsA, EzrA, SepF, SepH and ZipA. SepF participates in bundling and membrane anchoring of FtsZ in gram-positive bacteria. We report in vitro biophysical studies of the interactions between FtsZ and cytoplasmic component of cognate SepF from three different bacteria: Mycobacterium tuberculosis , Staphylococcus aureus and Enterococcus gallinarum. While the cytosolic domain of SepF from M. tuberculosis is a dimer, those from S. aureus and E. gallinarum polymerize to form ring-like structures. Mycobacterial SepF helps in bundling of FtsZ filaments to form thick filaments and large spirals. On the other hand, ring-forming SepF from the Firmicutes bundle FtsZ into tubules.

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Metadata

Authors
Choudhury, J., & Chaudhuri, B. N.
Affiliations
Institute of Microbial Technology (IN)
Corresponding author
Choudhury, Jagrity
ORCID
Venue
Cytoskeleton
Publisher
openRxiv
Published
2024-07-21
Volume
82
Issue
7
Pages
432-443
ISSN
1949-3584 (ISSN-L)
DOI
10.1101/2024.07.18.604210
OpenAlex
W4405653034
Cited by
3
References
1
Citations by year
2025: 3
FWCI
0.80
Type
journal article
Language
en
Open access
closed
Locations
  • Cytoskeleton (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
Funders
Council of Scientific and Industrial Research, India
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
SepF, FtsZ, Z-ring assembly, bacterial cell division, divisome, FtsZ polymerization, oligomeric state, Mycobacterium tuberculosis, Staphylococcus aureus, Enterococcus gallinarum
MeSH terms
Bacterial Proteins, Cytoskeletal Proteins, Mycobacterium tuberculosis, Staphylococcus aureus

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CoChat. (2026). Citation check: Tubules, rods and spirals: diverse modes of SepF-FtsZ assembling. https://app.cochat.ai/papers/10.1101/2024.07.18.604210
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Choudhury, J., & Chaudhuri, B. N. (2024). Tubules, rods and spirals: diverse modes of SepF-FtsZ assembling. Cytoskeleton. https://doi.org/10.1101/2024.07.18.604210

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