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PreprintCited by 4Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsDNA Repair Mechanisms

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The Spc105/Kre28 complex promotes mitotic error correction by outer kinetochore recruitment of Ipl1/Sli15

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Dudziak, Alexander, Pleuger, Richard, Schmidt, Jasmin, Hamm, Frederik, Tendulkar, Sharvari, Jänen, Karolin, Vetter, Ingrid R., Singh, Sylvia, Fischböck, Josef, Herzog, Franz, Westermann, Stefan·2024·The EMBO Journal
DOI 10.1101/2024.10.01.615642PMC PMC12170873

Preprint — published as 10.1038/s44318-025-00437-w · Paper page

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Abstract

Kinetochores link chromosomes to dynamic microtubules of the mitotic spindle. To ensure equal chromosome segregation, sister chromatids must achieve biorientation. The conserved kinase Aurora B phosphorylates outer kinetochore proteins on attachments lacking tension, allowing re-establishment of new connections until biorientation is achieved. Aurora B localizes to the centromere as part of the chromosomal passenger complex (CPC), but the underlying recruitment pathways can be eliminated without disrupting biorientation. It therefore remains unclear how the kinase operates during error correction. Here, we identify the conserved Spc105/Kre28 complex as an outer kinetochore receptor of the Aurora kinase Ipl1 and its activator Sli15 in Saccharomyces cerevisiae . We show that mutations in the helix bundle domain of Spc105/Kre28 impair mitotic error correction, resembling the effects of ipl1 or sli15 mutants. The defects can be suppressed by artificial recruitment of Ipl1. In biochemical experiments Ipl1/Sli15 directly associates with Spc105/Kre28, and a conserved segment in the Sli15 central domain is crucially involved in the binding mechanism. These results have important implications for the mechanism of tension-dependent error correction during chromosome biorientation.

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Research works printed but not linked: 65

  1. 10.1038/S41586-024-07487-W · DOI · Paper page
  2. 10.1038/nature09594 · DOI
  3. 10.1101/gad.934801 · DOI
  4. 10.1083/jcb.201905144 · DOI
  5. 10.1083/jcb.201306054 · DOI
  6. 10.1038/nature12057 · DOI
  7. 10.1016/j.cell.2008.03.020 · DOI
  8. 10.1038/s41467-022-29542-8 · DOI
  9. 10.1126/sciadv.adg7480 · DOI · Paper page
  10. 10.1101/gad.1126303 · DOI
  11. 10.1016/j.cell.2016.10.011 · DOI
  12. 10.1093/nar/gkv332 · DOI
  13. Evans R , O’Neill M , Pritzel A , Antropova N , Senior A , Green T , Žídek A , Bates R , Blackwell S , Yim J , et al (2022) Protein complex prediction with AlphaFold-Multimer. bioRxiv: 2021.2010.2004.463034 · DOI · Paper page
  14. 10.15252/msb.202211084 · DOI
  15. 10.7554/eLife.42879 · DOI
  16. 10.1016/j.cub.2019.03.051 · DOI
  17. 10.1016/j.cell.2008.09.046 · DOI
  18. 10.1016/j.celrep.2020.108190 · DOI
  19. 10.1083/jcb.201907087 · DOI
  20. 10.1126/science.1221483 · DOI
  21. 10.3389/fcell.2017.00112 · DOI
  22. 10.1083/jcb.201403081 · DOI
  23. 10.1126/science.1101366 · DOI
  24. 10.1016/j.cell.2007.07.045 · DOI
  25. 10.1126/science.1235532 · DOI
  26. 10.1093/bib/bbx108 · DOI · Paper page
  27. 10.3389/fonc.2015.00225 · DOI
  28. 10.1016/j.jmb.2007.05.022 · DOI
  29. 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B · DOI
  30. 10.1016/j.cub.2023.09.006 · DOI
  31. 10.1126/science.1167000 · DOI
  32. 10.1016/j.cub.2012.03.052 · DOI
  33. 10.1038/emboj.2012.356 · DOI
  34. 10.1016/j.devcel.2011.05.008 · DOI
  35. 10.1038/S41592-022-01488-1 · DOI · Paper page
  36. 2017, A Molecular View of Kinetochore Assembly and Function, Biology (Basel), 6, 5
  37. 10.1038/ncb3065 · DOI
  38. 10.1371/journal.pone.0007640 · DOI
  39. 10.1016/j.cub.2024.04.054 · DOI
  40. 10.1093/NAR/GKAB1038 · DOI · Paper page
  41. 10.1016/j.molcel.2022.04.027 · DOI
  42. 10.1016/j.cell.2016.10.005 · DOI
  43. 10.1016/j.molcel.2014.01.019 · DOI
  44. 10.1002/jcc.20084 · DOI
  45. 10.1016/j.cub.2024.03.062 · DOI · Paper page
  46. 10.1038/s41594-024-01230-9 · DOI
  47. 10.1016/j.cub.2011.04.011 · DOI
  48. 10.1098/rsob.210274 · DOI
  49. 10.1038/embor.2012.1 · DOI
  50. 10.1016/j.molcel.2005.03.031 · DOI
  51. 10.1016/j.cub.2012.03.051 · DOI
  52. 10.1016/j.cub.2018.08.038 · DOI
  53. 10.3390/cells11091462 · DOI
  54. 10.1073/pnas.1821945116 · DOI
  55. 10.1038/nmeth.2103 · DOI
  56. 10.1016/j.cell.2009.03.035 · DOI
  57. 10.1093/bioinformatics/btp033 · DOI
  58. 10.1038/nsmb1186 · DOI
  59. 10.1038/nature19333 · DOI
  60. 10.1083/jcb.200804170 · DOI
  61. 10.1101/gad.304782.117 · DOI
  62. 10.1038/ncb2515 · DOI
  63. 10.1126/SCIENCE.ABN3810/SUPPL_FILE/SCIENCE.ABN3810_MOVIES_S1_AND_S2.ZIP · DOI
  64. 10.1038/s41594-024-01249-y · DOI · Paper page
  65. 10.1016/j.cub.2024.04.067 · DOI

Metadata

Authors
Dudziak, A., Pleuger, R., Schmidt, J., Hamm, F., Tendulkar, S., Jänen, K., Vetter, I. R., Singh, S., Fischböck, J., Herzog, F., & Westermann, S.
Affiliations
  • University of Duisburg-Essen (DE)
  • Max Planck Institute of Molecular Physiology (DE)
  • Ludwig-Maximilians-Universität München (DE)
  • IMC University of Applied Sciences Krems (AT)
Corresponding author
Westermann, Stefan
ORCID
Venue
The EMBO Journal
Publisher
openRxiv
Published
2024-10-02
Volume
44
Issue
12
Pages
3492-3520
ISSN
0261-4189 (ISSN-L)
DOI
10.1101/2024.10.01.615642
PMC
PMC12170873
OpenAlex
W4409781585
Cited by
4
References
1
Citations by year
2026: 4
FWCI
2.00
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Universitätsbibliographie, Universität Duisburg-Essen (submittedVersion)
  • DuEPublico (University of Duisburg-Essen) (submittedVersion, cc-by)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
Deutsche Forschungsgemeinschaft (424228829)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
Aurora B kinase, outer kinetochore, chromosome biorientation, chromosomal passenger complex, kinetochore-microtubule attachment, protein-protein interaction, Saccharomyces cerevisiae
MeSH terms
Microtubule-Associated Proteins, Mitosis, Mutation, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Kinetochores, Cell Cycle Proteins, Chromosome Segregation, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins, Aurora Kinases

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