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Cited by 22Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsDNA Repair Mechanisms

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Microtubule end-on attachment maturation regulates Mps1 association with its kinetochore receptor

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Pleuger, Richard, Cozma, Christian, Hohoff, Simone, Denkhaus, Christian, Dudziak, Alexander, Kaschani, Farnusch, Kaiser, Markus, Musacchio, Andrea, Vetter, Ingrid R., Westermann, Stefan·2024·Current Biology
DOI 10.1016/j.cub.2024.03.062

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Abstract

Faithful chromosome segregation requires that sister chromatids establish bi-oriented kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) prevents premature anaphase onset with incomplete attachments. However, how microtubule attachment and checkpoint signaling are coordinated remains unclear. The conserved kinase Mps1 initiates SAC signaling by localizing transiently to kinetochores in prometaphase and is released upon bi-orientation. Using biochemistry, structure predictions, and cellular assays, we shed light on this dynamic behavior in Saccharomyces cerevisiae. A conserved N-terminal segment of Mps1 binds the neck region of Ndc80:Nuf2, the main microtubule receptor of kinetochores. Mutational disruption of this interface, located at the backside of the paired CH domains and opposite the microtubule-binding site, prevents Mps1 localization, eliminates SAC signaling, and impairs growth. The same interface of Ndc80:Nuf2 binds the microtubule-associated Dam1 complex. We demonstrate that the error correction kinase Ipl1/Aurora B controls the competition between Dam1 and Mps1 for the same binding site. Thus, binding of the Dam1 complex to Ndc80:Nuf2 may release Mps1 from the kinetochore to promote anaphase onset.

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Metadata

Authors
Pleuger, R., Cozma, C., Hohoff, S., Denkhaus, C., Dudziak, A., Kaschani, F., Kaiser, M., Musacchio, A., Vetter, I. R., & Westermann, S.
Affiliations
  • University of Duisburg-Essen (DE)
  • Max Planck Institute of Molecular Physiology (DE)
Corresponding author
Westermann, Stefan
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2024-06
Volume
34
Issue
11
Pages
2279-2293.e6
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/j.cub.2024.03.062
OpenAlex
W4398167221
Cited by
22
References
2
Citations by year
2026: 4 · 2025: 10 · 2024: 6
FWCI
4.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Universitätsbibliographie, Universität Duisburg-Essen (submittedVersion)
Funders
Deutsche Forschungsgemeinschaft (424228829, SFB1430)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
Mps1 kinase, spindle assembly checkpoint, kinetochore-microtubule attachment, Dam1 complex, kinetochore, chromosome segregation, Saccharomyces cerevisiae, checkpoint signaling
MeSH terms
Microtubule-Associated Proteins, Microtubules, Nuclear Proteins, Saccharomyces cerevisiae, Protein Serine-Threonine Kinases, Kinetochores, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins

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