CoChat
Sign Up
PreprintCited by 0RNA and protein synthesis mechanismsRNA Research and SplicingNuclear Structure and Function

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Composition and RNA binding specificity of metazoan RNase MRP

Open access
Liu, Yuan, He, Shiyang, Pyo, Anzie, Zheng, Shanshan, Chen, Meijuan, Cheloufi, Sihem, Slavov, Nikolai, Marzluff, William F, Murn, Jernej·2025·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2025.02.21.639568PMC PMC11870539

Preprint — published as 10.1093/nar/gkaf829 · Paper page

Open this paper in CoChat — free account

Abstract

Ribonuclease (RNase) MRP is a conserved RNA-based enzyme that is essential for maturation of ribosomal RNA (rRNA) in eukaryotes. However, the composition and RNA substrate specificity of this multisubunit ribonucleoprotein complex in higher eukaryotes remain a mystery. Here, we identify NEPRO and C18ORF21 as constitutive subunits of metazoan RNase MRP. Both proteins are specific to RNase MRP and are the only ones distinguishing this enzyme from the closely related RNase P, which selectively cleaves transfer RNA-like substrates. We find that NEPRO and C18ORF21 each form a complex with all other subunits of RNase MRP, stabilize its catalytic RNA, and are required for rRNA maturation and cell proliferation. We harness our discovery to identify a full suite of in vivo RNA targets of each enzyme, including positions of potential cleavage sites at nucleotide resolution. These findings resolve the general composition of metazoan RNase MRP, illuminate its RNA binding specificity, and provide valuable assets for functional exploration of this essential eukaryotic enzyme.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2025.02.21.639568

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 65

  1. 10.1002/wrna.1269 · DOI
  2. 10.1038/nsmb.2939 · DOI
  3. 10.1261/rna.2214510 · DOI
  4. 10.1016/j.jbc.2024.105729 · DOI
  5. 10.1016/S0092-8674(01)00205-7 · DOI
  6. 10.1038/sj.cr.7310120 · DOI
  7. 10.1016/S0968-0004(00)88962-8 · DOI
  8. 10.1126/science.abc0149 · DOI
  9. 10.1038/s41467-020-17308-z · DOI
  10. Lan P , et al. Structural insight into precursor tRNA processing by yeast ribonuclease P. Science 362, (2018). · DOI
  11. 10.1038/nature09516 · DOI
  12. 10.1016/j.cell.2018.10.003 · DOI
  13. 10.1093/nar/gkt432 · DOI
  14. 10.1017/S1355838201010469 · DOI
  15. 10.1101/gad.8.21.2617 · DOI
  16. 10.1074/jbc.M409568200 · DOI
  17. 10.1016/j.tree.2021.11.005 · DOI
  18. 10.1128/MCB.24.3.945-953.2004 · DOI
  19. 10.1074/jbc.M112.389023 · DOI
  20. 10.1002/wrna.1766 · DOI
  21. 10.1038/s41587-023-01773-0 · DOI · Paper page
  22. 10.1007/978-3-030-34436-8_6 · DOI
  23. 10.1002/ajmg.a.61267 · DOI
  24. 10.1111/dgd.12232 · DOI
  25. 10.1093/nar/gki756 · DOI
  26. 10.1038/nsmb.1838 · DOI · Paper page
  27. 10.1016/j.ymeth.2019.10.003 · DOI
  28. 10.1038/s41594-019-0300-4 · DOI
  29. 10.1016/0092-8674(83)90117-4 · DOI · Paper page
  30. 10.1016/j.cell.2008.10.012 · DOI
  31. 10.1101/gad.204438.112 · DOI
  32. 10.1002/j.1460-2075.1995.tb06986.x · DOI
  33. 10.1261/rna.2393711 · DOI
  34. 10.1101/gad.286963.116 · DOI
  35. 10.1016/j.biochi.2012.02.001 · DOI
  36. 10.1016/j.bcmd.2007.02.001 · DOI
  37. 10.1093/nar/gkq1050 · DOI
  38. 10.1038/s41467-020-18377-w · DOI · Paper page
  39. 10.1038/s41589-018-0021-8 · DOI · Paper page
  40. 10.1016/0014-4827(88)90126-7 · DOI
  41. 10.1016/0092-8674(92)90528-K · DOI
  42. 10.1083/jcb.201207131 · DOI
  43. 10.1091/mbc.E21-05-0251 · DOI
  44. 10.1074/jbc.RA119.009902 · DOI
  45. 10.1038/sj.onc.1202260 · DOI
  46. 10.1128/MCB.20.15.5516-5528.2000 · DOI
  47. 10.1016/j.tibs.2021.07.005 · DOI
  48. 10.1083/jcb.200512025 · DOI
  49. 10.1074/jbc.270.42.25281 · DOI
  50. 10.1093/nar/gkq701 · DOI
  51. 10.1002/wrna.9 · DOI
  52. 10.1038/s41467-022-28295-8 · DOI
  53. 10.1038/s41467-024-47449-4 · DOI
  54. 10.15252/msb.20145625 · DOI
  55. 10.1038/S41592-022-01488-1 · DOI · Paper page
  56. 10.1002/PRO.3943 · DOI
  57. 10.1016/j.ymeth.2019.11.008 · DOI
  58. 10.1093/bioinformatics/ btx330 · DOI
  59. 2012, FLEXBAR-Flexible Barcode and Adapter Processing for Next-Generation Sequencing Platforms, Biology (Basel, 1, 895
  60. 10.1093/bioinformatics/bts635 · DOI · Paper page
  61. 10.1101/gr.209601.116 · DOI
  62. 10.1186/s13059-017-1364-2 · DOI
  63. 10.1093/bioinformatics/btq033 · DOI · Paper page
  64. 10.1038/nbt.1754 · DOI · Paper page
  65. 10.1093/bib/bbs017 · DOI · Paper page

Metadata

Authors
Liu, Y., He, S., Pyo, A., Zheng, S., Chen, M., Cheloufi, S., Slavov, N., Marzluff, W. F., & Murn, J.
Affiliations
  • University of California, Riverside (US)
  • Northeastern University (US)
  • University of North Carolina at Chapel Hill (US)
Corresponding author
Murn, Jernej
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2025-02-23
DOI
10.1101/2025.02.21.639568
PMC
PMC11870539
OpenAlex
W4407869485
Cited by
0
References
2
Type
posted content
Language
en
Open access
Licence
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
  • Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion, cc-by)
  • Apollo (University of Cambridge) (submittedVersion, cc-by)
Funders
National Institute of General Medical Sciences (R01GM144693, R35GM151004, R35GM148218)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
RNase MRP, ribonuclease P, ribosomal RNA maturation, enzyme subunit composition, RNA binding specificity, ribozymes, cleavage site, cell proliferation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review