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PreprintCited by 1RNA Research and SplicingRNA and protein synthesis mechanismsGene Regulatory Network Analysis

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Homeostasis in the Central Dogma of Molecular Biology: the importance of mRNA instability

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Pérez-Ortín, José E., Tordera, Vicente, Chávez, Sebastián·2019·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/599050

Preprint — published as 10.1080/15476286.2019.1655352 · Paper page

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Abstract

Cell survival requires the control of biomolecule concentration, i.e. biomolecules should approach homeostasis. With information-carrying macromolecules, the particular concentration variation ranges depend on each type: DNA is not buffered, but mRNA and protein concentrations are homeostatically controlled, which leads to the ribostasis and proteostasis concepts. In recent years, we have studied the particular features of mRNA ribostasis and proteostasis in the model organism S. cerevisiae . Here we extend this study by comparing published data from three other model organisms: E. coli, S. pombe and cultured human cells. We describe how mRNA ribostasis is less strict than proteostasis. A constant ratio appears between the average decay and dilution rates during cell growth for mRNA, but not for proteins. We postulate that this is due to a trade-off between the cost of synthesis and the response capacity. This compromise takes place at the transcription level, but is not possible at the translation level as the high stability of proteins, versus that of mRNAs, precludes it. We hypothesize that the middle-place role of mRNA in the Central Dogma of Molecular Biology and its chemical instability make it more suitable than proteins for the fast changes needed for gene regulation. Graphical Abstract:

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Metadata

Authors
Pérez-Ortín, J. E., Tordera, V., & Chávez, S.
Affiliations
  • Universitat de València (ES)
  • Instituto de Biomedicina de Sevilla (ES)
Corresponding author
Pérez-Ortín, José E.
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2019-04-05
DOI
10.1101/599050
OpenAlex
W2932838414
Cited by
1
References
11
Citations by year
2021: 1
Type
posted content
Language
en
Open access
Locations
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
Funders
  • European Commission
  • Ministerio de Economía y Competitividad (BFU2016‐77728‐C3‐3‐P, BFU2016-77728-C3-1-P)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords
proteostasis, mRNA stability, mRNA degradation, protein stability, central dogma, gene expression regulation, response capacity, transcriptional regulation, Saccharomyces cerevisiae, Escherichia coli, Schizosaccharomyces pombe, human cells

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