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PreprintCited by 16Cellular Mechanics and InteractionsMaterial Dynamics and Properties3D Printing in Biomedical Research

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Compaction dynamics during progenitor cell self-assembly reveal granular mechanics

Open access
Smeets, Bart, Pešek, Jiří, Deckers, Thomas, Hall, Gabriella Nilsson, Cuvelier, Maxim, Ongenae, Steven, Bloemen, Veerle, Luyten, Frank P, Papantoniou, Ioannis, Ramon, Herman·2019·Matter
DOI 10.1101/699447

Preprint — published as 10.1016/j.matt.2020.02.016

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Abstract

We study the self-assembly dynamics of human progenitor cells in agarose micro-wells that are used for production of chondrogenic organoids. Using image analysis on time-lapse microscopy, we estimate the aggregate area in function of time for a large number of aggregates. In control conditions, the aggregate radius follows an exponential relaxation that is consistent with the dewetting dynamics of a liquid film. Introducing Y-27632 Rho kinase inhibitor, the compatibility with the liquid model is lost, and slowed down relaxation dynamics are observed. We demonstrate that these aggregates behave as granular piles undergoing compaction, with a density relaxation that follows a stretched exponential. Using simulations with an individual cell-based model, we construct a phase diagram of cell aggregates that suggests that the aggregate in presence of Rho kinase inhibitor approaches the glass transition.

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Metadata

Authors
Smeets, B., Pešek, J., Deckers, T., Hall, G. N., Cuvelier, M., Ongenae, S., Bloemen, V., Luyten, F. P., Papantoniou, I., & Ramon, H.
Affiliations
  • KU Leuven (BE)
  • FORTH Institute of Chemical Engineering Sciences (GR)
Corresponding author
Smeets, Bart
ORCID
Venue
Matter
Publisher
openRxiv
Published
2019-07-11
Volume
2
Issue
5
Pages
1283-1295
ISSN
2590-2385 (ISSN-L)
DOI
10.1101/699447
OpenAlex
W3012094454
Cited by
16
References
1
Citations by year
2026: 3 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 6 · 2021: 4
FWCI
1.37
Type
journal article
Language
en
Open access
Locations
  • Matter (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • Lirias (KU Leuven) (submittedVersion)
Funders
  • Fonds Wetenschappelijk Onderzoek (12Z6118N)
  • KU Leuven
  • Vlaamse regering
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Materials Science › Materials Chemistry › Material Dynamics and Properties
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
aggregate compaction, granular mechanics, Rho kinase inhibition, Y-27632, stretched exponential relaxation, glass transition, cell-based simulation, phase diagram, cell aggregates

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CoChat. (2026). Citation check: Compaction dynamics during progenitor cell self-assembly reveal granular mechanics. https://app.cochat.ai/papers/10.1101/699447
The paper
Smeets, B., Pešek, J., Deckers, T., Hall, G. N., Cuvelier, M., Ongenae, S., Bloemen, V., Luyten, F. P., Papantoniou, I., & Ramon, H. (2019). Compaction dynamics during progenitor cell self-assembly reveal granular mechanics. Matter. https://doi.org/10.1101/699447