The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
In vivo RNAi: Today and Tomorrow
Open accessAbstract
RNA interference (RNAi) provides a powerful reverse genetics approach to analyze gene functions both in tissue culture and in vivo. Because of its widespread applicability and effectiveness it has become an essential part of the tool box kits of model organisms such as Caenorhabditis elegans, Drosophila, and the mouse. In addition, the use of RNAi in animals in which genetic tools are either poorly developed or nonexistent enables a myriad of fundamental questions to be asked. Here, we review the methods and applications of in vivo RNAi to characterize gene functions in model organisms and discuss their impact to the study of developmental as well as evolutionary questions. Further, we discuss the applications of RNAi technologies to crop improvement, pest control and RNAi therapeutics, thus providing an appreciation of the potential for phenomenal applications of RNAi to agriculture and medicine.
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Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Initial sequencing and comparative analysis of the mouse genome
Mouse Genome Sequencing Consortium (2002) · Nature · doi:10.1038/nature01262 · Cited by 7,187
RNAi-mediated gene silencing in non-human primates
Zimmermann, T. S., Lee, A. C. H., Akinc, A., Bramlage, B., Bumcrot, D., Fedoruk, M. N., Harborth, J., Heyes, J. A., Jeffs, L. B., John, M., et al. (2006) · Nature · doi:10.1038/nature04688 · Cited by 1,357 · cited 1×
“Delivery is currently considered the biggest hurdle as the introduction of siRNAs systemically into body fluids can result in their degradation, off-target effects, and immune detection and subsequent reactions (see for example Zimmermann et al. 2006).”
BackgroundHigh-throughput RNAi screening in cultured cells: a user's guide
Echeverri, C. J., & Perrimon, N. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1836 · Cited by 380 · cited 3×
“Four different types of RNAi reagents are used for in vivo studies: synthetic siRNAs, small hairpin RNAs (shRNAs), small hairpin microRNAs (shmiRNAs), and long dsRNAs (reviews by Echeverri and Perrimon 2006; Lee and Kumar 2009) (Fig.”
Self-citationGenome-wide analysis of Notch signalling in Drosophila by transgenic RNAi
Mummery-Widmer, J. L., Yamazaki, M., Stoeger, T., Novatchkova, M., Bhalerao, S., Chen, D., Dietzl, G., Dickson, B. J., & Knoblich, J. A. (2009) · Nature · doi:10.1038/nature07936 · Cited by 305
Cited by
Adaptation of High-Throughput Screening in Drug Discovery—Toxicological Screening Tests
Szymański, P., Markowicz, M., & Mikiciuk-Olasik, E. (2011) · International Journal of Molecular Sciences · doi:10.3390/ijms13010427 · Cited by 454
New genes as drivers of phenotypic evolution
Chen, S., Krinsky, B. H., & Long, M. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3521 · Cited by 428
RNAi screening comes of age: improved techniques and complementary approaches
Mohr, S. E., Smith, J. A., Shamu, C. E., Neumüller, R. A., & Perrimon, N. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3860 · Cited by 332
Self-citationShukla, J. N., Kalsi, M., Sethi, A., Narva, K. E., Fishilevich, E., Singh, S., Mogilicherla, K., & Palli, S. R. (2016) · RNA Biology · doi:10.1080/15476286.2016.1191728 · Cited by 309
Cech, T. R. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a006742 · Cited by 244
Metadata
- Authors
- Perrimon, N., Ni, J.-Q., & Perkins, L.
- Venue
- Cold Spring Harbor Perspectives in Biology
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2010-06-09
- Volume
- 2
- Issue
- 8
- Pages
- a003640-a003640
- ISSN
- 1943-0264 (ISSN-L)
- OpenAlex
- W2100581989
- Cited by
- 205
- References
- 5
- Type
- posted content
- Language
- en
- Open access
- bronze
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